Recombinant Mouse VSTM2B Fc Chimera Protein, CF

Recombinant Mouse VSTM2B Fc Chimera (Catalog # 10076-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 2-10 μg/mL.

2 μg/lane of Recombinant Mouse VSTM2B Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 61-70 kDa and 120-140 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse VSTM2B Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 2-10 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse VSTM2B protein
  

  Mouse VSTM2B (Thr29-Ser263) Accession # Q9JME9	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9JME9
• N-terminal Sequence: Thr29
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 61-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VSTM2B Fc Chimera Protein, CF

• V-set and transmembrane domain-containing protein 2B
• VSTM2B

Background

V-set and transmembrane domain-containing protein 2B, also known as VSTM-2B, is a single pass Type I membrane protein. Mouse VSTM-2B contains an N-Terminal signal peptide (1-28 aa), an extracellular domain (aa 29-263) which contains a single Ig-like V-Type domain (aa 29-143) with a single disulfide bond between C49 and C127, a helical transmembrane domain (aa 264-284) and a single histidine in the cytoplasm (aa 285). VSTM2B is expressed in multiple organs, with the highest expression in the pyloric antrum. The extracellular domain shares 89% and 96% sequence identity for the human and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits anti-CD3 induced IL-2 and IFN-g secretion on human T cells.

Bioinformatics

• Uniprot:
  • Q9JME9()