Recombinant Mouse Soggy-1/DkkL1 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Soggy-1/DkkL1 Protein Summary

• Details of Functionality: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 2-6 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Soggy-1/DkkL1 protein
  Leu21-Leu230, with a C-terminal 10-His tag
• Accession #: AAF02679
• N-terminal Sequence: Leu21
• Structure / Form: monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Dkkl1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Soggy-1/DkkL1 Protein

• Cancer/testis antigen 34
• CT34dickkopf-like 1 (soggy)
• dickkopf-like 1
• DkkL1
• Protein soggy-1
• SGY
• SGY1
• SGY-1dickkopf-like protein 1
• SGY1soggy
• Soggy1
• Soggy-1

Background

Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The mouse Soggy-1 cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence (2). Mouse Soggy-1 shares 65% and 90% aa sequence identity with human and rat Soggy-1, respectively, and approximately 15% aa sequence identity with mouse Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3 - 5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).

1. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
2. Krupnik, V.E. et al. (1999) Gene 238:301.
3. Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
4. Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
5. Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
6. Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.

Bioinformatics

• Gene Symbol: Dkkl1
• Uniprot:
  • AAF02679()