Recombinant Mouse SLITRK3 His-tag Protein, CF

2 μg/lane of Recombinant Mouse SLITRK3 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 94-105 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SLITRK3 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse SLITRK3 protein
  Thr30-Ser655, with a C-terminal 6-His tag
• Accession #: Q810B9
• N-terminal Sequence: Thr30
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 94-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SLITRK3 His-tag Protein, CF

• MGC138681
• SLIT and NTRK-like family, member 3
• SLITRK3

Background

SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature mouse SLITRK3 contains 6 leucine rich repeats (LRR), a C‑terminal LRR domain, and 6 more LRR flanked by a pair of N- and C‑terminal LRR domains (2, 3). Within the ECD, mouse SLITRK3 shares 98% and 99.5% amino acid sequence identity with human and rat SLITRK3, respectively. SLITRK3 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-6). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is up-regulated during the development of gastrointestinal stromal tumors (7).

1. Ko, J. (2012) Mol. Cells 34:335.
2. Aruga, J. et al. (2003) Gene 315:87.
3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
6. Loh, K. H. et al. (2016) Cell 166:1295.
7. Wang, C.J. et al. (2015) World J. Gastroenterol. 21:8398.

Bioinformatics

• Uniprot:
  • Q810B9()