Recombinant Mouse Protocadherin-12 Protein, CF

Images

 
There are currently no images for Protocadherin-12 (7926-CA).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Protocadherin-12 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.15 - 0.9 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Protocadherin-12 protein
Leu18-Ala716, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained, Leu18 predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Protein/Peptide Type
Recombinant Proteins
Gene
Pcdh12
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
77 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-105 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Protocadherin-12 Protein, CF

  • PCDH12
  • protocadherin 12
  • Protocadherin12
  • Protocadherin-12
  • Vascular cadherin-2
  • vascular endothelial cadherin 2
  • Vascular endothelial cadherin-2
  • VECAD2
  • VE-cad-2
  • VE-cadherin 2
  • VE-cadherin-2
  • VE-cadherin-2protocadherin-12

Background

Protocadherin-12 (PCDH12; also called VE-Cadherin-2) is an approximately 150 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins. These are type I transmembrane proteins that exhibit calcium-dependent but relatively weak homophilic adhesion (1-4). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1). Within the ECD, mouse Protocadherin-12 shares 95% and 82% amino acid sequence identity with rat and human Protocadherin-12, respectively. Protocadherin-12 is expressed in mouse endothelial, glycogen-rich trophoblast, and mesangial cells where it clusters at intercellular junctions (1, 2). It may be important during placental development, as placentas from Protocadherin-12-deficient mice display altered morphogenesis and transcription profiles (3). In humans, the ECD of Protocadherin-12 can be shed via proteolytic cleavage (5). It circulates at increased levels in sera from pregnant women exhibiting pre-eclampsia compared to women with nonpathological gestations (5).
  1. Telo’, P. et al. (1998) J. Biol. Chem. 273:17565.
  2. Rampon, C. et al. (2005) Exp. Cell Res. 302:48.
  3. Rampon, C. et al. (2008) Physiol. Genomics 34:193.
  4. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.
  5. Bouillot, S. et al. (2011) J. Biol. Chem. 286:15195.

Customers Who Viewed This Item Also Viewed...

AF1002
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-53235
Species: Hu
Applications: Flow, ICC/IF, IHC-P, PAGE
NBP1-81410
Species: Hu
Applications: IHC, IHC-P
NB600-1081
Species: Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-55658
Species: Hu
Applications: ICC/IF
NBP1-84565
Species: Hu
Applications: IHC, IHC-P
MAB7278
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-82120
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF761
Species: Mu
Applications: WB, Simple Western, Flow, IHC, AdBlk, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-46403
Species: Hu
Applications: IHC
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB2240
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-38535
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB8626
Species: Hu
Applications: Flow, CyTOF-ready

Publications for Protocadherin-12 (7926-CA) (0)

There are no publications for Protocadherin-12 (7926-CA).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protocadherin-12 (7926-CA) (0)

There are no reviews for Protocadherin-12 (7926-CA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Protocadherin-12 (7926-CA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Protocadherin-12 (7926-CA)

Discover related pathways, diseases and genes to Protocadherin-12 (7926-CA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Protocadherin-12 (7926-CA)

Discover more about diseases related to Protocadherin-12 (7926-CA).
 

Pathways for Protocadherin-12 (7926-CA)

View related products by pathway.

PTMs for Protocadherin-12 (7926-CA)

Learn more about PTMs related to Protocadherin-12 (7926-CA).

Blogs on Protocadherin-12

There are no specific blogs for Protocadherin-12, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Protocadherin-12 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Pcdh12
Entrez
Uniprot