Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF

When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 1 µg/mL, Recombinant Mouse Betatrophin Fc Chimera (Catalog # 9983-AN)binds with an ED50 of 0.12‑0.72 μg/mL.

2 μg/lane of Recombinant Mouse Angiopoietin-like Protein 8/Betatrophin was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 52-56 kDa and 105-110 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Angiopoietin-like Protein 8/Betatrophin Fc Chimera binds with an ED₅₀ of 0.12-0.72 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 8/Betatrophin protein
  

  Mouse Betatrophin (Ala22-Ala198) Accession # Q8R1L8	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q8R1L8
• N-terminal Sequence: Ala22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 52-56 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS,NaCl and CHAPS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF

• Angiopoietin like Protein 8
• Angiopoietin-like Protein 8
• ANGPTL8
• Betatrophin
• C19orf80
• chromosome 19 open reading frame 80
• Gm6484
• hepatocellular carcinoma-associated gene TD26
• hepatocellular carcinoma-associated protein TD26
• Lipasin
• PRO1185
• PVPA599
• RIFL
• TD26
• UNQ599

Background

Betatrophin (also called ANGPTL8, lipasin and RIPL), a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. It is most homologous to ANGPTL3 but lacks the fibrinogen-like domain of ANGPTL3 and other ANGPTL family members (1). Mouse Betatrophin is 183 amino acids (aa) in length and shares 72% aa identity with human Betatrophin. Betatrophin is a crucial modulator in lipid metabolism. It can form a complex with the N-terminal of ANGPTL3, and the complex is necessary for inhibition of LPL and triglyceride modulation (2). Betatrophin did not control beta-cell expansion in the mouse model (3). In addition to the lipid and glucose metabolism, ANGPTL8 has been reported to be involved in many other disorders (4).

1. Quagliarini, F. et al. (2012) PNAS 109(48):19751.
2. Chi, X., et al. (2017) Molecular Metabolism 6:1137.
3. Cox A.R.  et al. PLoS One (2016) 11:e0159276.
4. Luo, M. et al. (2018) Front Endocrino 9:169.

Bioinformatics

• Uniprot:
  • Q8R1L8()