Recombinant Human VSTM2B His-tag Protein, CF

Recombinant Human VSTM2B (Catalog # 10061-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.

2 μg/lane of Recombinant Human VSTM2B was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 37 - 45 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human VSTM2B His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 1-6 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human VSTM2B protein
  Ala29-Thr263, with a C-terminal 6-His tag
• Accession #: A6NLU5
• N-terminal Sequence: Ala29
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 37-45 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VSTM2B His-tag Protein, CF

• V-set and transmembrane domain-containing protein 2B
• VSTM2B

Background

VSTM2B, or V-set and transmembrane domain-containing protein 2B is a membrane protein which includes a 235 amino acid (aa) extracellular domain that contains an Ig-like V-type domain, a 21 aa transmembrane domain, and one aa in cytoplasm. This extracellular domain shares 45% aa identity human VSTM2A. The mature extracellular domain of human VSTM2B shares 86% and 85% aa sequence identity for the mouse and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits the human T cell activation, including anti-CD3 induced IL-2, IFN-gamma secretion, and T cell proliferation.

Bioinformatics

• Uniprot:
  • A6NLU5()