Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave benzyloxycarbonyl-Arg-thiobenzyl ester (Z-R-SBzl). The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human Tryptase epsilon/BSSP-4 protein
Ala33-Ser317 & Val50-Ser317, both with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ala33 & Val50
Structure / Form
Pro and mature forms
Protein/Peptide Type
Recombinant Enzymes
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Multiple bands between 33-38 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 50 mM Tris, 0.05% (w/v) Brij-35, pH 8.0
  • Recombinant Human Tryptase  epsilon /BSSP‑4 (rhBSSP-4) (Catalog # 5047-SE)
  • Substrate: Z-Arg-SBzl (MP Biochemicals, Catalog # SB001)
  • DTNB (5,5’-dithibis(2-nitrobenzoic acid)) (Sigma, Catalog # D-8130)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhBSSP-4 to 0.5 ng/µL in Aassay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer containing 200 µM DTNB.
  3. Load 50 µL of 0.5 ng/µL rhBSSP-4 into a plate, and start the reaction by adding 50 µL of 200 µM Substrate to wells. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
  4. Read at an absorbance of 405 nm for 5 minutes in kinetic mode.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/M
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank

      **Using the extinction coefficient 13260 M-1cm-1

      ***Using the path correction 0.32 cm

     Note: the output of many spectrophotometers is in mOD

Per Well:
  • rhBSSP-4: 0.025 µg
  • Substrate: 100 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF

  • BSP-2
  • BSSP4
  • BSSP-4
  • hBSSP-4
  • MGC9599
  • prosemin
  • protease, serine S1 family member 22
  • protease, serine, 22
  • PRSS22
  • serine protease 22
  • serine protease 26
  • SP001LA
  • Tryptase epsilon
  • UNQ302/PRO343


Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha 1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase  epsilon efficiently converts pro‑urokinase‑type plasminogen activator to its mature, active form (3).

  1. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
  2. Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
  3. Yasuda, S. et al. (2005) Blood 105:3893.

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Gene Symbol PRSS22