Recombinant Human SLITRK3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SLITRK3 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human SLITRK3 protein
  Thr29-Val651, with substitutions of Arg425Ser, Asn490Tyr, Phe510Leu and a C-terminal 6-His tag
• Accession #: O94933
• N-terminal Sequence: Thr29
• Protein/Peptide Type: Recombinant Proteins
• Gene: SLITRK3
• Purity: >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-108 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SLITRK3 Protein, CF

• MGC138681
• SLIT and NTRK-like family, member 3
• SLITRK3

Background

SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK3 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK3 shares 98% amino acid sequence identity with mouse and rat SLITRK3. It is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-5). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is upregulated during the development of gastrointestinal stromal tumors (6).

1. Ko, J. (2012) Mol. Cells 34:335.
2. Aruga, J. et al. (2003) Gene 315:87.
3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
6. Wang, C.-J. et al. (2015) World J. Gastroenterol. 21:8398.

Bioinformatics

• Gene Symbol: SLITRK3
• Uniprot:
  • O94933()