Recombinant Human Protocadherin-12 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Protocadherin-12 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.2-1.0 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Protocadherin-12 protein
  Gln25-Ser705, with a C-terminal 6-His tag
• Accession #: AAH52973
• N-terminal Sequence: No results obtained.Gln25 inferred from enzymatic pyroglutamate treatment revealing Glu26
• Protein/Peptide Type: Recombinant Proteins
• Gene: PCDH12
• Purity: >90%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Protocadherin-12 Protein, CF

• PCDH12
• protocadherin 12
• Protocadherin12
• Protocadherin-12
• Vascular cadherin-2
• vascular endothelial cadherin 2
• Vascular endothelial cadherin-2
• VECAD2
• VE-cad-2
• VE-cadherin 2
• VE-cadherin-2
• VE-cadherin-2protocadherin-12

Background

Protocadherin-12 (PCDH12; also called VE-Cadherin-2) is an approximately 150 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins. These are type I transmembrane proteins that exhibit calcium-dependent but relatively weak homophilic adhesion (1-4). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1). Within the ECD, human Protocadherin-12 shares 83% and 82% amino acid sequence identity with mouse and rat Protocadherin-12, respectively. Protocadherin-12 is expressed in mouse endothelial,
glycogen-rich trophoblast, and mesangial cells where it clusters at intercellular junctions (1, 2). It may be important during placental development, as placentas from Protocadherin-12-deficient mice display altered morphogenesis and transcription profiles (3). In humans, the ECD of Protocadherin-12 can be shed via proteolytic cleavage (5). It circulates at increased levels in sera from pregnant women exhibiting pre-eclampsia compared to women with nonpathological gestations (5).rich

1. Telo’, P. et al. (1998) J. Biol. Chem. 273:17565.
2. Rampon, C. et al. (2005) Exp. Cell Res. 302:48.
3. Rampon, C. et al. (2008) Physiol. Genomics 34:193.
4. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.
5. Bouillot, S. et al. (2011) J. Biol. Chem. 286:15195.

Bioinformatics

• Gene Symbol: PCDH12
• Uniprot:
  • AAH52973()