Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The ED50 for this effect is 5-30 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human PD-ECGF/Thymidine Phosphorylase protein
Ala11-Gln482
Accession #
N-terminal Sequence
Ala11
Protein/Peptide Type
Recombinant Proteins
Gene
TYMP
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
229-PE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein

  • dThdPase
  • EC 2.4.2
  • EC 2.4.2.4
  • ECGF
  • ECGF1
  • ECGF1TP
  • endothelial cell growth factor 1 (platelet-derived)
  • Gliostatin
  • hPD-ECGF
  • MEDPS1
  • MNGIE
  • MTDPS1
  • PDECGF
  • PD-ECGF
  • Platelet-derived endothelial cell growth factor
  • TdRPase
  • Thymidine Phosphorylase
  • TPase
  • TYMP

Background

PD-ECGF, also known as TPase and gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5-fluorouracil and its derivatives. The human PD-ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The latter protein was purified from quiescent astrocytes and described as an astrocyte and astrocytoma cell growth inhibitor based on its ability to inhibit 3H-thymidine incorporation.

  1. Haraguchi, M. et al. (1994) Nature 368:198.
  2. Toi, M. et al. (2005) Lancet Oncol. 6:158.
  3. Akiyama, S. et al. (2004) Canc. Sci. 95:851.

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Publications for PD-ECGF/Thymidine Phosphorylase (229-PE)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioassay
(2)
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(2)
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Bioinformatics

Gene Symbol TYMP
Uniprot