Recombinant Human ILDR1 Fc Chimera Protein, CF

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2 μg/lane of Recombinant Human ILDR1 Fc Chimera(Catalog # 10152-D1) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 40-49 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human ILDR1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human ILDR1 Fc Chimera is immobilized at 2.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human ILDR2 Fc Chimera that produces 50% of the optimal binding response is 2-12 μg/mL
Source
Human embryonic kidney cell, HEK293-derived human ILDR1 protein
Human ILDR1
(Leu24-His167)
Accession # Q86SU0
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Leu24
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-49 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ILDR1 Fc Chimera Protein, CF

  • Angulin-2
  • autosomal recessive 42
  • DFNB42
  • ILDR1
  • ILDR1alpha
  • ILDR1alpha'
  • ILDR1beta
  • immunoglobulin-like domain containing receptor 1
  • immunoglobulin-like domain-containing receptor 1 alpha
  • immunoglobulin-like domain-containing receptor 1 alpha'
  • immunoglobulin-like domain-containing receptor 1 beta

Background

Immunoglobulin-like domain containing receptor 1, or ILDR1, is a single pass Type I membrane protein that belongs to the immunoglobulin superfamily. Human ILDR1 contains a signal peptide (aa 1-23), an Ig-like V-type extracellular domain (aa 24-167), a helical transmembrane domain amino acids (aa168-188), and a cytoplasmic domain (aa 189-546) that contains a cysteine rich region and an arginine rich region (1). The extracellular domain of human ILDR1 shares a 96.5% homology with mouse and rat respectively. ILDR1 is mainly expressed in the prostate, with lower levels also found in testis, pancreas, heart and liver (1). ILDR1 has also been found expressed by intestinal cholecystokinin (CCK)-producing cells (2). ILDR1 has three splice variants. Two of those are expressed on the cell surface, while one is expressed in the cytoplasm, except when co-expressed with one of the other two splice variants. The cytoplasmic variant is the shortest transcript and is not found in healthy tissue, but only in lymphoma samples (1). It is suggested that ILDR1 plays a role in regulating intestinal hormone levels by stimulating release of CCK in response to fatty acids (2). Loss of function of ILDR1 is associated with Autosomal-Recessive Hearing Impairment (3). Our data shows that ILDR1 binds to human immunoglobulin-like domain containing receptor 2 (ILDR2).
  1. Hauge, H. et al. (2004) Biochem. Biophys. Res. Commun. 323:970.
  2. Chandra, R. et al. (2013) J. Clin. Invest. 123:3343.
  3. Borck, G. et al. (2011) Am. J. Hum. Genet. 88:127.

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