Recombinant Human DPP9 Protein, CF

Images

 
There are currently no images for DPP9 (5419-SE).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human DPP9 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >750 pmol/min/μg, as measured under the described conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DPP9 protein
Arg2-Leu892 with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
No result obtained
Protein/Peptide Type
Recombinant Enzymes
Gene
DPP9
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
92 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 25 mM Tris, pH 8.0
  • Recombinant Human DPP9 (rhDPP9) (Catalog # 5419-SE)
  • Substrate: H-Gly-Pro-AMC (Bachem, Catalog # I-1225) Prepare a 10 mM stock in DMSO.
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhDPP9 to 1 ng/µL in Assay Buffer.
  2. Dilute substrate to 200 µM in Assay Buffer.
  3. Load 50 µL of 1 ng/µL rhDPP9 into a plate, and start the reaction by adding 50 µL of 50 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 5 minutes.
  5. Calculate specific activity:
     Specific Activity (pmol/min/µg) = Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).

Per Well:
  • rhDPP9: 0.050 µg
  • Substrate: 100 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DPP9 Protein, CF

  • dipeptidyl peptidase 9
  • Dipeptidyl peptidase IV-related protein 2
  • dipeptidyl peptidase IV-related protein-2
  • Dipeptidyl peptidase IX
  • Dipeptidyl peptidase-like protein 9
  • dipeptidylpeptidase 9
  • dipeptidyl-peptidase 9
  • DKFZp762F117
  • DP9
  • DPLP9
  • DPP IX
  • DPP9
  • DPRP2
  • DPRP-2
  • EC 3.4.14.5
  • FLJ16073

Background

DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).

  1. Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
  2. Qi, S.Y. et al. (2003) Biochem. J. 373:179.
  3. Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
  4. Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
  5. Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
  6. Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.

Customers Who Viewed This Item Also Viewed...

AF1180
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP2-01830
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P
AF3715
Species: Hu
Applications: WB, IHC, IP
NBP2-16669
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88502
Species: Hu
Applications: IHC, IHC-P
NBP1-84986
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91612
Species: Mu
Applications: WB
NBP1-49879
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-64843
Species: Mu
Applications: Flow, IHC, IHC-Fr, IHC-P
NBP1-32421
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-87404
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-46535
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, IHC, IHC-Fr, IHC-P
NBP2-33950
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01249
Species: Hu
Applications: WB, Flow, IHC, IHC-P

Publications for DPP9 (5419-SE) (0)

There are no publications for DPP9 (5419-SE).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DPP9 (5419-SE) (0)

There are no reviews for DPP9 (5419-SE). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for DPP9 (5419-SE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human DPP9 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol DPP9
Uniprot