RARRES1 Antibody [Unconjugated]

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RARRES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4255) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RARRES1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Product Discontinued

RARRES1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human RARRES1
  Pro43-Phe294
  Accession # P49788
• Specificity: Detects human RARRES1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RARRES1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RARRES1 Antibody [Unconjugated]

• RARRES1
• RAR-responsive protein TIG1
• retinoic acid receptor responder (tazarotene induced) 1
• retinoic acid receptor responder protein 1
• TIG1
• TIG1Tazarotene-induced gene 1 protein

Background

RARRES1 (retinoic acid receptor responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Human RARRES1 is 294 amino acids (aa) in length. It is a type II transmembrane (TM) protein with a TM segment between aa 21-42. Three potential splice variants exist. There is a 46 aa N terminal extension, a four aa substitution for aa 225-294 and a combination of these two variants. Over aa 43-294, human RARRES1 shares 75% aa identity with mouse RARRES1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RARRES1 Antibody (AF4255)(5)

Publications using AF4255

• Anqun Chen, Ye Feng, Han Lai, Wenjun Ju, Zhengzhe Li, Yu Li et al. Soluble RARRES1 induces podocyte apoptosis to promote glomerular disease progression Journal of Clinical Investigation 9/14/2020 [PMID: 32634130]
• H Huebner, R Strick, DL Wachter, S Kehl, PL Strissel, R Schneider-, A Hartner, W Rascher, LC Horn, MW Beckmann, M Ruebner, FB Fahlbusch Hypermethylation and loss of retinoic acid receptor responder 1 expression in human choriocarcinoma J. Exp. Clin. Cancer Res., 2017-11-23;36(1):165. 2017-11-23 [PMID: 29169400] (Western Blot, Human)
• Hui-Ling Chen, Haeri Seol, Kristy Jean Brown, Heather Gordish-Dressman, Ashley Hill, Vittorio Gallo et al. Secretome Survey of Human Plexiform Neurofibroma Derived Schwann Cells Reveals a Secreted form of the RARRES1 Protein International Journal of Molecular Sciences 7/24/2012 [PMID: 22942771]
• Peng Z, Shen R, Li YW Epigenetic repression of RARRES1 is mediated by methylation of a proximal promoter and a loss of CTCF binding. PLoS ONE, 2012-05-17;7(5):e36891. 2012-05-17 [PMID: 22615834] (Western Blot, Human)
• Sahab ZJ, Hall MD, Me Sung Y, Dakshanamurthy S, Ji Y, Kumar D, Byers SW Tumor Suppressor RARRES1 Interacts with Cytoplasmic Carboxypeptidase AGBL2 to Regulate the alpha-Tubulin Tyrosination Cycle. Cancer Res., 2011-02-08;71(4):1219-28. 2011-02-08 [PMID: 21303978] (Western Blot, Human)

Review for RARRES1 Antibody (AF4255) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Tingting Li ICC Human 05/01/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: SUM-149 xenografts mice
• Species: Human

Bioinformatics

• Gene Symbol: RARRES1
• Uniprot:
  • P49788()