RAMP1 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

RAMP1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human RAMP1
  Cys27-Ser117
  Accession # O60894
• Specificity: Detects human RAMP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse RAMP1 is observed and less than 1% cross-reactivity with recombinant human (rh) RAMP2 and rhRAMP3 is observe
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RAMP1 Antibody [Alexa Fluor® 350]

• calcitonin receptor-like receptor activity modifying protein 1
• Calcitonin-receptor-like receptor activity-modifying protein 1
• CRLR activity-modifying protein 1
• RAMP1
• receptor (calcitonin) activity modifying protein 1
• receptor (G protein-coupled) activity modifying protein 1
• receptor activity modifying protein 1
• receptor activity-modifying protein 1

Background

RAMP1 (Receptor Activity Modifying Protein 1) is a 14-18 kDa member of the RAMP family of proteins. Variability in MW is due to the absence, or presence, of intramolecular disulfide bonds that form in the ER and Golgi. It is expressed on/in neurons, vascular endothelial cells, visceral and vascular smooth muscle cells, mammary epithelium, osteoblasts and cardiomyocytes. RAMP1 interacts with CRLR/CLR to form an 84 kDa noncovalent receptor complex for CGRP and AM, and with CTR to form a 76 kDa receptor complex for amylin. Mature human RAMP1 is a 122 amino acid (aa) nonglycosylated type I transmembrane protein that contains a 91 aa extracellular domain (ECD) (aa 27-117) plus a ten aa cytoplasmic tail. In the ECD, residues 78-90 bind AM, while residues 91-103 bind CGRP. In the absence of CRLR and CTR, RAMP1 will form 30-32 kDa disulfide-linked homodimers in the ER/Golgi. Over aa 27-117, human RAMP1 shares 65% aa identity with mouse RAMP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O60894(Human)