PRMT2 Antibody

Western Blot: PRMT2 Antibody [NB110-93593] - Detection of PRMT2 in Jurkat whole cell lysates using NB110-93593.

Immunocytochemistry/Immunofluorescence: PRMT2 Antibody [NB110-93593] - PRMT2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

PRMT2 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human PRMT2 (within residues 380-433). [Swiss-Prot# P55345]
• Specificity: This is specific for PRMT2, and may also recognize a homodimer at ~98 kDa as referenced in Chao et al (3).
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRMT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:200
  • Western Blot 2 ug/ml
• Application Notes: This PRMT2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where bands are observed at ~47 kDa and ~98 kDa. In ICC/IF diffuse cytoplasmic staining with some nuclear reactivity was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PRMT2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRMT2 Antibody

• EC 2.1.1.-
• EC 2.1.1.125
• Histone-arginine N-methyltransferase PRMT2
• HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1
• HMT1 hnRNP methyltransferase-like 1 (S. cerevisiae)
• HMT1 hnRNP methyltransferase-like 1
• HMT1
• HRMT1L1PRMT2 beta
• MGC111373
• PRMT2 alpha
• PRMT2 gamma
• protein arginine methyltransferase 2
• protein arginine N-methyltransferase 2

Background

PRMT2 is a member of the protein arginine N-methyltransferase family that has been shown to be both an androgen receptor (AR)-associated coactivator and an estrogen receptor. PRMT2 expression is upregulated in the lung under hypoxic conditions, and has been shown to inhibit cell activation and promote programmed cell death through a NF-kappaB-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRMT2
• Entrez:
  • 3275(Human)
• Uniprot:
  • P55345(Human)