PRMT1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

PRMT1 Antibody [Alexa Fluor® 594] Summary

• Specificity: Detects human and mouse PRMT1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRMT1 Antibody [Alexa Fluor® 594]

• ANM1
• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.77
• HCP1
• Histone-arginine N-methyltransferase PRMT1
• HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
• HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
• HMT1
• HMT2
• HRMT1L2
• HRMT1L2HMT1 hnRNP methyltransferase-like 2
• Interferon receptor 1-bound protein 4
• IR1B4
• IR1B4protein arginine N-methyltransferase 1
• PRMT1
• protein arginine methyltransferase 1

Background

PRMT/PRMT1 (Protein-Arginine Methyltransferase 1; also HRMT1L2 and HMT2) is a 42‑45 kDa type I member of the arginine N‑methyltransferase family of enzymes. It is ubiquitously expressed and dimethylates single arginine residue nitrogen atoms in a distributive manner (i.e.-with substrate release following each methlyation step). PRMT1 forms homodimers, and heterodimers with PRMT3, and exists as part of a 300‑400 kDa complex in vivo. Human PRMT1 is 361 amino acids in length and contains one adenosyl-L-methionine binding site (aa 85‑153) and a phosphorylation site at Tyr299. There are multiple splice variants that show distinct substrate specificities. There is an alternate start site 10 aa upstream of the standard start site, and a single Met, plus a five aa and 11 aa substiuition for aa 1‑19. One isoform has a 2 aa substitution for aa 1‑20 accompanied by a deketion of aa 52‑67. Over aa 218‑361, human PRMT1 is 100% identical in aa sequence to mouse PRMT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.