PRMT1 Antibody (779) - Azide and BSA Free

Western Blot: PRMT1 Antibody (779) [NBP2-43744] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT1 Antibody (779) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744] - PRMT1 Antibody (779) detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. PRMT1 stained by PRMT1 Antibody (779) diluted at 1:200.

Western Blot: PRMT1 Antibody (779) [NBP2-43744] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE PRMT1 antibody [779] dilution: 1:500

Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744] - PRMT1 Antibody (779) detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. PRMT1 stained by PRMT1 Antibody (779) diluted at 1:200.

PRMT1 antibody [GT779] detects PRMT1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PRMT1 protein stained by PRMT1 antibody [GT779] (NBP2-43744) diluted at 1:200.Blue: Hoechst 33342 staining.

PRMT1 antibody [GT779] detects PRMT1 protein by western blot analysis.A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug Raw264.7 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract 10% SDS-PAGEPRMT1 antibody [GT779] (NBP2-43744) dilution: 1:1000The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 779
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PRMT1 Antibody (779) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PRMT1 Antibody (779) - Azide and BSA Free (NBP2-43744) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PRMT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRMT1. The exact sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRMT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:1000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for PRMT1 Antibody (779) - Azide and BSA Free

• ANM1
• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.77
• HCP1
• Histone-arginine N-methyltransferase PRMT1
• HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
• HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
• HMT1
• HMT2
• HRMT1L2
• HRMT1L2HMT1 hnRNP methyltransferase-like 2
• Interferon receptor 1-bound protein 4
• IR1B4
• IR1B4protein arginine N-methyltransferase 1
• PRMT1
• protein arginine methyltransferase 1

Background

PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRMT1 Antibody (NBP2-43744)(1)

Publication using NBP2-43744

• Lavogina D, Nasirova N, SOrmus T et al. Conjugates of adenosine mimetics and arginine-rich peptides serve as inhibitors and fluorescent probes but not as long-lifetime photoluminescent probes for protein arginine methyltransferases Journal of peptide science : an official publication of the European Peptide Society 2022-10-08 [PMID: 36208424]

Bioinformatics

• Gene Symbol: PRMT1