pre-mRNA cleavage factor I (59 kDa subunit) Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Ma, Pm, Mu, Rt, Ca, Pm, Eq, Gp, Pm-Or, RM
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

pre-mRNA cleavage factor I (59 kDa subunit) Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human cleavage and polyadenylation specificity factor 7, 59 kDa subunit using the numbering given in entry NP_079087.2 (GeneID 79869).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Equine (100%), Rhesus Macaque (100%), Orangutan (100%), Guinea Pig (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPSF7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with White-tufted-ear marmoset, Crab-eating Macaque, Small-eared Galago and Northern White-cheeked Gibbon. Mammal reactivity reported in scientific literature (PMID: 27180817). Primate reactivity reported in scientific literature (PMID: 24147125).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for pre-mRNA cleavage factor I (59 kDa subunit) Antibody [CoraFluor™ 1]

• CFIm59
• cleavage and polyadenylation specific factor 7, 59kDa
• CPSF 59 kDa subunit
• FLJ12529,59 kDa subunit
• FLJ39024
• MGC9315
• pre-mRNA cleavage factor I, 59 kDa subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPSF7