PLBD1 Overexpression Lysate (Native)

Images

 
Western Blot: PLBD1 Overexpression Lysate (Adult Normal) [NBL1-10759] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLBD1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PLBD1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209501 and based on accession number NM_024829. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens phospholipase B domain containing 1 (PLBD1), mRNA.
Gene
PLBD1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
63.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PLBD1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PLBD1 Overexpression Lysate (Native)

  • EC 3.1.1
  • EC 3.1.1.-
  • FLJ22662
  • LAMA-like protein 1
  • Lamina ancestor homolog 1
  • phospholipase B domain containing 1
  • Phospholipase B domain-containing protein 1
  • PLB homolog 1
  • putative phospholipase B-like 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-53110
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-13396
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2564
Species: Hu
Applications: WB
NBP1-76900
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP2-45844
Species: Hu
Applications: WB, IHC
NBP1-79488
Species: Hu
Applications: WB
AF644
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NBL1-10759
Species: Hu
Applications: WB

Publications for PLBD1 Lysate (NBL1-10759) (0)

There are no publications for PLBD1 Lysate (NBL1-10759).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PLBD1 Lysate (NBL1-10759) (0)

There are no reviews for PLBD1 Lysate (NBL1-10759). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PLBD1 Lysate (NBL1-10759) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PLBD1 Products

Bioinformatics Tool for PLBD1 Lysate (NBL1-10759)

Discover related pathways, diseases and genes to PLBD1 Lysate (NBL1-10759). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Research Areas for PLBD1 Lysate (NBL1-10759)

Find related products by research area.

Blogs on PLBD1

There are no specific blogs for PLBD1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

PLBD1 Antibody
H00079887-D01P

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PLBD1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PLBD1