Pescadillo Antibody

Product Discontinued

Pescadillo Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 475 and 525 of mouse Pescadillo using the numbering given in entry NP_075027.1 (GeneID 64934).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PES1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2500
  • Immunoprecipitation
  • Western Blot 1:500-1:5000
• Application Notes: Immunofluorescence and Western Blot By WB this antibody recognizes bands around 75 kDa representing Pescadillo. Nuclear extracts are recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pescadillo Antibody

• pescadillo homolog 1, containing BRCT domain (zebrafish)
• pescadillo homolog
• PESpescadillo (zebrafish) homolog 1, containing BRCT domain

Background

Pescadillo is a BRCT (BRCA1 C-terminal) domain protein and plays a role in DNA synthesis, ribosome genesis, cell proliferation, and transformation. Alternate names for Pescadillo include PES1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pescadillo Antibody (NB100-242)(3)

Publications using NB100-242

• Sikorski EM, Uo T, Morrison RS et al. Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells. J Biol Chem 2006-08-01 [PMID: 16816389]
• Maiorana A, Tu X, Cheng G et al. Role of pescadillo in the transformation and immortalization of mammalian cells. Oncogene 2004-09-01 [PMID: 15273728]
• Lerch-Gaggl A, Haque J, Li J et al. Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. J Biol Chem 2002-11-01 [PMID: 12237316]

Bioinformatics

• Gene Symbol: PES1
• Entrez:
  • 64934(Mouse)
• Uniprot:
  • NP_075027.1(Mouse)