Penicillinase Antibody [Biotin]

Dot Blot: Penicillinase Antibody [Biotin] [NB100-2006] - Dot Blot results of Rabbit Penicillinase antibody [Biotin].Dots are Penicillinase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: Rabbit Anti-Penicillinase Biotin at 1ug/mL for 1hr at RT.Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30min at RT.

Dot Blot results of Rabbit Penicillinase Antibody [Biotin]. Dots are Penicillinase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 30 min at RT. Primary Antibody: Rabbit Anti-Penicillinase Biotin at 1ug/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP

• Reactivity: Ba
• Applications: WB, DB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin
• Concentration: LYOPH

Penicillinase Antibody [Biotin] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae]
• Immunogen: Penicillinase [E.cloacae] (Uniprot: P05364)
• Localization: Cytoplasmic
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Penicillinase [E.clocae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: This product has been tested by dot blot and is suitable to be assayed against 1.0 ug of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Reactive with Penicillinase from E. cloacae.
Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Penicillinase Antibody [Biotin]

• Aer 1
• Aer1
• Beta lactamase 1
• Beta lactamase AER 1
• Beta lactamase AER1
• Beta lactamase type I
• Beta lactamase
• Bla L
• Bla
• BlaL
• OXA 9
• OXA9
• Oxacillinase
• PenPC

Background

Penicillinase is a beta-lactamase, showing specificity for penicillins by hydrolysing the beta-lactam ring. Beta lactamases play a significant role in the high resistance of certain organinsms to penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Penicillinase resistant beta lactams have been developed, such as methicillin, although there is widespread resistance to these now also (MRSA). This antibody is useful for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P05364(Bacteria)