Penicillinase Antibody

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Penicillinase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Penicillinase (Enterobacter cloacae).
Localization
Cytoplasmic
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Penicillinase [E.clocae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Penicillinase [E.cloacae] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:7,000 - 1:21,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross-reacts with Enterobacter cloacae.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for Penicillinase Antibody

  • Aer 1
  • Aer1
  • Beta lactamase 1
  • Beta lactamase AER 1
  • Beta lactamase AER1
  • Beta lactamase type I
  • Beta lactamase
  • Bla L
  • Bla
  • BlaL
  • OXA 9
  • OXA9
  • Oxacillinase
  • PenPC

Background

Penicillinase is a beta-lactamase, showing specificity for penicillins by hydrolysing the beta-lactam ring. Beta lactamases play a significant role in the high resistance of certain organinsms to penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Penicillinase resistant beta lactams have been developed, such as methicillin, although there is widespread resistance to these now also (MRSA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional Penicillinase Products

Penicillinase NB600-647

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