PD-ECGF/Thymidine Phosphorylase Antibody (826722) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 826722
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

PD-ECGF/Thymidine Phosphorylase Antibody (826722) [Alexa Fluor® 350] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PD-ECGF/Thymidine Phosphorylase
  Met1-Pro471
  Accession # Q99N42
• Specificity: Detects mouse PD-ECGF/Thymidine Phosphorylase in direct ELISAs. Detects human, mouse, and rat PD-ECGF/Thymidine Phosphorylase in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-ECGF/Thymidine Phosphorylase Antibody (826722) [Alexa Fluor® 350]

• dThdPase
• EC 2.4.2
• EC 2.4.2.4
• ECGF
• ECGF1
• ECGF1TP
• endothelial cell growth factor 1 (platelet-derived)
• Gliostatin
• hPD-ECGF
• MEDPS1
• MNGIE
• MTDPS1
• PDECGF
• PD-ECGF
• Platelet-derived endothelial cell growth factor
• TdRPase
• Thymidine Phosphorylase
• TPase
• TYMP

Background

TYMP (Thymidine phosphorylase/TP; also [PD]-ECGF/platelet-derived endothelial cell growth factor, gliostatin and TdRPase) is a 50-55 kDa member of the pyrimidine-nucleoside phosphorylase family of enzymes. TYMP/TP is both a cytosolic and secreted molecule that is expressed by a variety of cell types, including, macrophages, hepatocytes, endometrial gland epithelium, vascular smooth muscle and endothelial cells. It has also been found in select tumor cell types, and via an angiogenic activity, has been proposed to promote tumor growth. TYMP converts thymidine to thymine and 2-deoxy-ribose-1P, and it is the 2-deoxy-ribose component that is believed to promote endothelial cell migration (but not proliferation). This may be due to the fact that 2-deoxy-ribose induces reactive oxygen species, which drive the production of angiogenic factors, and that 2-deoxy-ribose also activates endothelial cell integrins. Mouse TYMP is 471 amino acids (aa) in length. It contains a phosphorylase domain (aa 96-348), followed by a C-terminal region (aa 374-448). Although TYMP circulates, there is no definitive signal sequence. TYMP is known to form homodimers. Full-length mouse TYMP (aa 1-471) shares 80% and 92% aa sequence identity with human and rat TYMP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.