PCBP3 Antibody

Western Blot: PCBP3 Antibody [NBP2-19716] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCBP3 antibody [N1C2] diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PCBP3 Antibody [NBP2-19716] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.

Western Blot: PCBP3 Antibody [NBP2-19716] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: PCBP3 Antibody [NBP2-19716] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCBP3 antibody [N1C2] diluted at 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PCBP3 Antibody Summary

• Description: Novus Biologicals Rabbit PCBP3 Antibody (NBP2-19716) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PCBP3. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCBP3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PCBP3 Antibody

• Alpha-CP3
• poly (rC)-binding protein 310ALPHA-CP3
• poly(rC) binding protein 3
• poly(rC)-binding protein 3

Background

This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PCBP3
• Uniprot:
  • P57721(Human)