Human p130Cas/BCAR1 ELISA Kit (Colorimetric)

ELISA: Human p130Cas/BCAR1 ELISA Kit (Colorimetric) [NBP2-69941] - Samples were spiked with high concentrations of Human p130Cas/BCAR1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human p130Cas/BCAR1 ELISA Kit (Colorimetric) [NBP2-69941] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human p130Cas/BCAR1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human BCAR1 in samples. No significant cross-reactivity or interference between Human BCAR1 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.68%
• Intra-Assay: CV% < 5.02%
• Spike Recovery: 83-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BCAR1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human p130Cas/BCAR1 ELISA Kit (Colorimetric)

• BCAR1
• breast cancer estrogen resistance 1
• Cas scaffolding protein family member 1
• CAS1
• CASFLJ45059
• CASS1
• CASS1CRKASbreast cancer estrogen resistance protein 1
• CRKAS
• Crk-associated substrate p130Cas
• CRK-associated substrate
• FLJ12176
• p130Cas

Background

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for p130Cas/BCAR1 ELISA Kit (NBP2-69941). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BCAR1