Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody

Product Discontinued

Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody Summary

• Immunogen: Synthetic peptide derived from CHRNA3 protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHRNA3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot 1:100-1:1000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 26061781)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody

• cholinergic receptor, nicotinic, alpha 3
• cholinergic receptor, nicotinic, alpha polypeptide 3
• CHRNA3
• LNCR2
• MGC104879
• NACHRA3
• neuronal acetylcholine receptor subunit alpha-3
• neuronal nicotinic acetylcholine receptor, alpha3 subunit
• Nicotinic Acetylcholine R alpha 3
• Nicotinic Acetylcholine Ra3
• PAOD2

Background

The nicotinic acetylcholine receptor (nAChR) is a ligand gated ion channel that mediates neurotransmission at the neuromuscular junction, autonomic ganglia and at some sites in the central nervous system. Distinct nAChR subtypes exist that can be stimulated by the neurotransmitter acetylcholine, the natural product nicotine, or by synthetic compounds. After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 3 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (NBP1-18793)(2)

Publications using NBP1-18793

• Kim CS, Choi JS, Joo SY et al. Nicotine-Induced Apoptosis in Human Renal Proximal Tubular Epithelial Cells. PLoS ONE. 2016-03-31 [PMID: 27028622] (WB, Human)
• Gonzalez-Islas C, Angel Garcia-Bereguiain M, O'Flaherty B, Wenner P. Tonic nicotinic transmission enhances spinal GABAergic presynaptic release and the frequency of spontaneous network activity Dev Neurobiol. 2015-06-09 [PMID: 26061781] (WB, Chicken)

FAQs for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (NBP1-18793). (Showing 1 - 1 of 1 FAQs).

1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
   • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Bioinformatics

• Gene Symbol: CHRNA3
• Entrez:
  • 1136(Human)
  • 110834(Mouse)
  • 25101(Rat)
• Uniprot:
  • P32297(Human)