MT-ND1 Antibody

Product Discontinued

MT-ND1 Antibody Summary

• Immunogen: ND1 (-, 21 a.a. ~ 72 a.a) partial recombinant protein with GST tag.MLTERKILGYIQLRKGPNVVGPYGLLQPFADAIKLFTKEPLKPATSTITLY
• Specificity: ND1 - NADH dehydrogenase subunit 1
• Clonality: Polyclonal
• Host: Mouse
• Gene: ND1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Unpurified antisera so the specific antibody concentration is unknown. Contains 50% glycerol.
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MT-ND1 Antibody

• EC 1.6.5.3
• mitochondrially encoded NADH dehydrogenase 1
• MTND1
• NAD1
• NADH dehydrogenase subunit 1
• NADH dehydrogenase, subunit 1 (complex I)
• NADH1
• ND1NADH dehydrogenase 1

Background

NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7),while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 1 (ND1) binds rotenone and rotenone analogs and might be involved in electron transfer to ubiquinone. Mutations in the ND1 gene may be implicated in several disorders, including Leber hereditary optic neuropathy, Alzheimer's disease and Parkinson disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MT-ND1 Antibody (H00004535-A01)(4)

Publications using H00004535-A01

• Martinez-Zamora A, Meseguer S, Esteve JM et al. Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier. PLoS One 2015-01-01 [PMID: 26642043] (WB)
• Rafiq J, Duno M, Ostergaard E et al. Exercise Intolerance and Myoglobinuria Associated with a Novel Maternally Inherited MT-ND1 Mutation. JIMD Rep 2015-06-25 [PMID: 26108648]
• Chuang YL, Hsu CY., et al. Changes in mitochondrial energy utilization in young and old worker honeybees (Apis mellifera). Age (Dordr) 2012-11-20 [PMID: 23179252]
• Karamanlidis G, Nascimben L, Couper GS et al. Defective DNA Replication Impairs Mitochondrial Biogenesis In Human Failing Hearts. Circ Res 2010-03-25 [PMID: 20339121]

Review for MT-ND1 Antibody (H00004535-A01) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 01/20/2015

Summary

• Application: Western Blot
• Sample Tested: Guinea pig lung, liver and heart
• Species: Other

Bioinformatics

• Gene Symbol: ND1
• Entrez:
  • 4535(Human)
• OMIM:
  • 516000(Human)