MT-ATP6 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: MT-ATP6 Antibody [NBP3-05579] - Immunofluorescence analysis of L929 cells using MT-ATP6 Polyclonal Antibody (NBP3-05579) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: MT-ATP6 Antibody [NBP3-05579] -analysis of various lysates, using MT-ATP6 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.

Product Discontinued

MT-ATP6 Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MT-ATP6 (YP_003024031.1). PPLLIPTSKYLINNR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for MT-ATP6 Antibody - Azide and BSA Free

• ATP synthase F0 subunit 6
• ATP6
• ATPase subunit 6
• ATPase6
• MTATP6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Publications for MT-ATP6 Antibody (NBP3-05579)(1)

Publication using NBP3-05579

• Gu X, Li K, Zhang M et al. Aspartyl-tRNA synthetase 2 orchestrates iron-sulfur metabolism in hematopoietic stem cells via fine-tuning alternative RNA splicing Cell reports 2023-10-31 [PMID: 37838946] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: ATP6