MFAP3 Antibody (1059216) [Unconjugated]

MFAP3 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human MFAP3 Monoclonal Antibody (Catalog # MAB10798) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1059216
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MFAP3 Antibody (1059216) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human MFAP3
  Leu22-Met147
  Accession # P55082
• Specificity: Detects human MFAP3 in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MFAP3 Antibody (1059216) [Unconjugated]

• DKFZp586F0219
• MFAP3
• microfibril-associated glycoprotein 3
• microfibrillar-associated protein 3

Background

Microfibril-associated glycoprotein 3 (MFAP3), is a member of the MFAP family which play a role in microfibril assembly, elastinogenesis, and tissue homeostasis. The family members were identified as potential components of fibrillin-containing microfibrils, but they do not share structural similarities (1). MFAP3 is found in ocular zonules. Human MFAP3 is a serine-rich acidic protein located in zonular fibers and consists of an extracellular domain (ECD) containing one Ig-like C2-type domain, a single transmembrane domain, and a cytoplasmic domain. Within the ECD, mature human MFAP3 shares 74% amino acid sequence identity with mouse and rat MFAP3. Among MFAP3-related pathways are degradation of the extracellular matrix and elastic fiber formation. MFAP is a candidate gene for heritable diseases affecting microfibrils (2).

1. Gibson, M.A. Landes Bioscience https://www.ncbi.nlm.nih.gov/books/NBK6448/.
2. Abrams W.R. (1995) Genomics 26:47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P55082()