MCHR1 Antibody (1032149) [Unconjugated]

Western blot shows lysates of human hypothalamus tissue, mouse hypothalamus tissue, and rat hypothalamus tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB79381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MCHR1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

MCHR1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB79381) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 1032149
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MCHR1 Antibody (1032149) [Unconjugated] Summary

• Immunogen: E. coli-derived human MCHR1
  Met1-Pro113
  Accession # Q99705
• Specificity: Detects human MCHR1 in direct ELISAs. Detects human, mouse, and rat MCHR1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCHR1 Antibody (1032149) [Unconjugated]

• G protein-coupled receptor 24
• GPR24
• GPR24SLC-1
• G-protein coupled receptor 24
• MCH receptor 1
• MCH1R
• MCH-1R
• MCH1RMCHR-1
• MCHR1
• MCH-R1
• melanin-concentrating hormone receptor 1
• MGC32129
• SLC1
• SLC1MCHR
• Somatostatin receptor-like protein

Background

MCHR1 (Melanin-concentrating Hormone Receptor-1), also called GPR24 (G-protein Coupled Receptor 24) or SLC1 (Somatostatin Receptor-like 1), is an approximately 44 kDa, seven-transmembrane receptor for MHC. It is most highly expressed in the brain but is also present in peripheral tissues. Engagement of MHCR1 has effects on food intake, energy homeostasis, anxiety and depression, such that rodents treated with small molecule antagonists weigh less and show reduced anxiety and depression in model systems than untreated rodents. Human MCHR1 shares 74% amino acid (aa) sequence identity with mouse MCHR1 between aa 1-113, and 82% with rat MCHR1 between aa 71-113.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q99705(Human)