MAGEA12 Overexpression Lysate

Images

 
Western Blot: MAGEA12 Overexpression Lysate (Adult Normal) [NBL1-12800] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAGEA12.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MAGEA12 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201480 and based on accession number NM_005367. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens melanoma antigen family A, 12 (MAGEA12), mRNA.
Gene
MAGEA12

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAGEA12 Overexpression Lysate

  • Cancer/testis antigen 1.12
  • CT1.12MAGE12F antigen
  • MAGE-12 antigen
  • MAGE12cancer/testis antigen family 1, member 12
  • melanoma antigen family A, 12
  • melanoma-associated antigen 12

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Other Available Formats

NBL1-12800

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Bioinformatics

Gene Symbol MAGEA12