![Western Blot: LSM12 Antibody [NBP3-12550] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa](http://images.novusbio.com/fullsize2/LSM12-Antibody-Western-Blot-NBP3-12550-img0001.jpg "Western Blot: LSM12 Antibody [NBP3-12550] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | E. coli-derived Human LSM12 fragment |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LSM12 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH7.0 |
| Preservative | 0.03% Proclin 300 |
| Purity | Protein A purified |
![Western Blot: MCT1 Antibody (2F9) [NBP2-03626] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCT1.](http://images.novusbio.com/images/MCT1-Antibody-(2F9)-Western-Blot-NBP2-03626-img0001.jpg)
![Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCT1 mouse monoclonal antibody.](http://images.novusbio.com/images/MCT1-Antibody-(2F9)-Immunohistochemistry-Paraffin-NBP2-03626-img0002.jpg)
![Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MCT1 mouse monoclonal antibody.](http://images.novusbio.com/images/MCT1-Antibody-(2F9)-Immunohistochemistry-Paraffin-NBP2-03626-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CCDC72 Antibody [NBP2-14594] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli & nuclear bodies.](http://images.novusbio.com/images2/CCDC72-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14594-img0003.jpg)
![Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/PABP-Antibody-(10E10)-Flow-Cytometry-NB120-6125-img0006.jpg)
![Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7.](http://images.novusbio.com/images2/PABP-Antibody-(10E10)-Western-Blot-NB120-6125-img0002.jpg)
![Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.](http://images.novusbio.com/images2/PABP-Antibody-(10E10)-Western-Blot-NB120-6125-img0003.jpg)
![Western Blot: EDC3 Antibody [NBP2-30473] - Analysis in human cell line HEK 293.](http://images.novusbio.com/images2/EDC3-Antibody-Western-Blot-NBP2-30473-img0004.jpg)
![Immunohistochemistry-Paraffin: EDC3 Antibody [NBP2-30473] - Staining of human testis shows moderate cytoplasmic positivity in subsets of cells in seminiferus ducts and Leydig cells.](http://images.novusbio.com/images2/EDC3-Antibody-Immunohistochemistry-Paraffin-NBP2-30473-img0001.jpg)
![Western Blot: EDC3 Antibody [NBP2-30473] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/images2/EDC3-Antibody-Western-Blot-NBP2-30473-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | LSM12 |
![Immunocytochemistry/Immunofluorescence: LSM12 Antibody [NBP3-12550] - Staining of LSM12 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LSM12 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize2/LSM12-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12550-img0002.jpg "Immunocytochemistry/Immunofluorescence: LSM12 Antibody [NBP3-12550] - Staining of LSM12 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LSM12 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunoprecipitation: LSM12 Antibody [NBP3-12550] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-LSM12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-LSM12 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa](http://images.novusbio.com/fullsize2/LSM12-Antibody-Immunoprecipitation-NBP3-12550-img0003.jpg "Immunoprecipitation: LSM12 Antibody [NBP3-12550] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-LSM12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-LSM12 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa")
![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP1-90063] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/Ataxin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90063-img0009.jpg)
![Western Blot: Ataxin-2 Antibody [NBP1-90063] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/Ataxin-2-Antibody-Western-Blot-NBP1-90063-img0016.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/images2/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90063-img0023.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")