LRRC8A Antibody - BSA Free

Western Blot: LRRC8A Antibody [NBP2-32158] - LRRC8A Antibody [NBP2-32158] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody NBP2-32158 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: LRRC8A Antibody [NBP2-32158] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody NBP2-32158 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated LRRC8A, NBP2-32158 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LRRC8A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LRRC8A Antibody - BSA Free (NBP2-32158) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-LRRC8A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 760 to 810 of human Leucine Rich Repeat Containing 8 Family, Member A using the numbering given in entry NP_001120716.1 (GeneID 56262).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRC8A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRRC8A Antibody - BSA Free

• AGM5
• FLJ10337
• FLJ41617
• KIAA1437
• KIAA1437leucine rich repeat containing 8
• leucine rich repeat containing 8 family, member A
• leucine-rich repeat-containing protein 8A
• LRRC8
• LRRC8A
• SWELL1

Background

The LRRC8A gene encodes a protein belonging to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. This family member is a putative fou

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRC8A Antibody (NBP2-32158)(3)

Publications using NBP2-32158

• Jahn M, Lang V, Rauh O et al. The Volume-Regulated Anion Channel LRRC8 is Involved in the Initiation of Epidermal Differentiation and is Deregulated in Psoriasis JID Innovations 2025-05-01 [PMID: 40492178]
• Ding Y, Hu L, Wang X Et al. The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE Neurobiol Dis 2022-11-04 [PMID: 36332813] (IHC-Fr, Mouse)
  
  Details:
  Citation using the DyLight 650 version of this antibody.
• Jahn M, Rauh O, Fauth T, Buerger C. Cell Volume Regulation in the Epidermis Cellular Physiology and Biochemistry 2021-02-18 [PMID: 33596608] (Immunohistochemistry)

Bioinformatics

• Gene Symbol: LRRC8A
• Uniprot:
  • NP_001120716.1(Human)