LRGUK Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: LRGUK Antibody [NBP2-17163] - The localization of LRGUK (red) in purified round spermatids, scale bar = 2um. Acrosomes were visualised by PNA (green). DAPI in blue. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005090) licensed under a CC-BY license.

Western Blot: LRGUK Antibody [NBP2-17163] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FLJ32786 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: LRGUK Antibody [NBP2-17163] - BT483 xenograft, using FLJ32786 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: LRGUK Antibody [NBP2-17163] - 30 ug Huh7 whole cell lysate/extract 7. 5 % SDS-PAGE antibody [N2C1], Internal dilution: 1:1000.

Western Blot: LRGUK Antibody [NBP2-17163] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FLJ32786 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: LRGUK Antibody [NBP2-17163] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Western Blot: LRGUK Antibody [NBP2-17163] - LRGUK-1 binds to HOOK2.(A) Growth of yeast carrying wild type LRGUK-1 (LRGUKWT/pDEST32) & HOOK2 (HOOK2/pDEST22) compared to those carrying LRGUKKaos (LRGUKKaos/pDEST32) & HOOK2 (HOOK2/pDEST22), & empty pDEST32 & pDEST22 vectors. Binding was absent (i.e. no growth) when empty vectors were used as a negative control. (B) Confirmation of the interaction between LRGUK-1 & HOOK2 by immunoprecipitation from HEK293 cells expressing EGFP-tagged LRGUK-1. EGFP-tagged LRGUK-1 was immunoprecipitated using GFP antibody-conjugated beads (anti-GFP-Trap-A beads), & immunoblotting was performed using HOOK2 antibody to detect the interaction between the EGFP-tagged LRGUK-1 & endogenous HOOK2. pEGFP-C1 represents a negative control which over-expressed EGFP protein only. Input: whole cell lysates of transfected HEK293 cells. (C) The localization of HOOK2 (red) in WT round spermatids, co-labelled with PNA (green) to mark the acrosome. HOOK2 was present within the developing acrosome/acroplaxome (arrowhead), at a region consistent with the leading edge of the acrosome & acroplaxome (arrows) & at a site consistent with the basal body (asterisk). (D) The localisation of HOOK2 (red) onto the microtubules ( alpha -tubulin, green) of the manchette in WT & LrgukKaos/Kaos elongating spermatids. Scale bar = 5 μm. (E) HOOK2 (red) within caudal epididymal sperm. Acetylated (Ac) tubulin was used as an axoneme marker. The asterisk indicates HOOK2 in the sperm basal body. Scale bar = 5 μm. In all images, nuclei are labelled with DAPI (blue). Please see S3 Fig. for negative control images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25781171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LRGUK Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human FLJ32786. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRGUK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25781171). Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for LRGUK Antibody - BSA Free

• FLJ32786
• leucine-rich repeat and guanylate kinase domain-containing protein
• leucine-rich repeats and guanylate kinase domain containing

Background

The LRGUK gene encodes a 825 amino acid long, 93 kDA leucine-rich repeat and guanylate kinase domain-containing protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for LRGUK Antibody (NBP2-17163)(1)

Publication using NBP2-17163

• Liu Y, DeBoer K, de Kretser DM et al. LRGUK-1 Is Required for Basal Body and Manchette Function during Spermatogenesis and Male Fertility. PLoS Genet. 2015-03-01 [PMID: 25781171] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: LRGUK
• Uniprot:
  • Q96M69(Human)