LPAR4/LPA4 Antibody (561203) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) Human LPAR4 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human LPAR4 Monoclonal Antibody (Catalog # MAB10217) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 561203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LPAR4/LPA4 Antibody (561203) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived transfected with human LPAR4/LPA₄
  Accession # Q99677
• Specificity: Detects human LPAR4/LPA₄ in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LPAR4/LPA4 Antibody (561203) [Unconjugated]

• G protein-coupled receptor 23
• GPCR23
• GPR23
• GPR23P2Y purinoceptor 9
• LPA4LPA-4
• LPAR4
• lysophosphatidic acid receptor 4
• P2RY9
• P2RY9G-protein coupled receptor 23
• P2Y5-like receptor
• P2Y5-LIKE
• P2Y9
• P2Y9LPA receptor 4
• Purinergic receptor 9

Background

Lysophosphatidic acid receptor 4, also known as LPA4, P2Y purinoceptor 9 (P2RY9) or GPR23, is a protein that in humans is encoded by the LPAR4 gene. LPA4 is a G protein-coupled receptor (GPCR) that binds the lipid signaling molecule lysophosphatidic acid (LPA) and mediates diverse cellular activities. Most LPA receptors share similarities with members of the S1P1/Edg family. GPA4, originally named P2Y9/GPR23, has been described as a fourth LPA receptor, LPA4, that together with LPA5 and LPA6 form a subfamily of LPA receptors structurally distant from the Edg family.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q99677()