LIR-8/CD85c/LILRB5 Antibody (1058033) [Unconjugated]

LILRB5/CD85c/LIR-8 was detected in immersion fixed Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line using Mouse Anti-Human LILRB5/CD85c/LIR-8 Monoclonal Antibody (Catalog # MAB30651) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1058033
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LIR-8/CD85c/LILRB5 Antibody (1058033) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line, NS0-derived human LILRB5/CD85c/LIR-8
  Arg18-His456
  Accession # O75023
• Specificity: Detects human LILRB5/CD87c/LIR-8 in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIR-8/CD85c/LILRB5 Antibody (1058033) [Unconjugated]

• CD85 antigen-like family member C
• CD85c antigen
• CD85c
• Leukocyte immunoglobulin-like receptor 8
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRB5
• LIR8
• LIR-8
• LIR8CD85C
• LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5
• member 5

Background

LIR8, also known as CD85c and LILRB5, belongs to the Leukocyte immunoglobulin-like receptors (LILR) family of transmembrane glycoproteins involved in regulating immune responses (1,2). There are at least thirteen LILR family members and are divided into activating (LILRA) or inhibiting (LILRB) molecules (1, 2). Mature human LIR8 consists of an extracellular domain (ECD) with four Ig-like domains, a transmembrane segment, and a cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM). Alternative splicing of human LIR8 generates at least 2 isoforms, one lacking the second Ig-like domain. The LILR family appears to be primate-specific receptors in terms of sequence homology. LIR8 is expressed on NK cells and in the tryptic granules of mast cells and negatively regulates immune cell activation (3, 4) It is present on the mast cell surface following cell activation and degranulation (4). Activated mast cells may also release soluble forms of LIR8 (3). LIR8 has also been shown to be expressed on T cells and induce CD8+ T cell proliferation (5). Consistent with the demonstrated binding of LILRB2 to Angiopoietin-like 2 and 5 (6), R&D Systems in-house testing indicates that LIR8 binds to Angiopoietin-like 7. Recently, a common missense variant of LIR8 was found to be associated with statin intolerance and myalgia (7).

1. Brown, D. et al. (2004) Tissue antigens. 64:215.
2. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
3. Borges, L. et al. (1997) J. Immunol. 159:5192.
4. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
5. Hogan, H. E. et al. (2016) Sci Rep. 6:21780.
6. Zheng, J. et al. (2012) Nature 485:656.
7. Siddigui, M. et al. (2017) Eur. Heart J. 38:3569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O75023()