LARGE Antibody

• Reactivity: Hu, Mu, Rt, Bv
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LARGE Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence RYTASSQRERESLE-C corresponding to N-Terminus (near) according to NP_004728.1.
• Epitope: RYTASSQRERESLE - N Terminus (near)
• Specificity: Reported variants represent identical protein: NP_004728.1, NP_598397.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LARGE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 125 kDa band in human kidney, pancreas and placenta lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 88.1 kDa band according to NP_004728.1. The 125 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LARGE Antibody

• Acetylglucosaminyltransferase-like 1A
• acetylglucosaminyltransferase-like protein
• glycosyltransferase-like protein LARGE1
• LARGE
• LARGE1
• like-acetylglucosaminyltransferase
• like-glycosyltransferase
• MDC1D
• MDDGA6
• MDDGB6
• xylosyl- and glucuronyltransferase LARGE1

Background

The LARGE gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LARGE Antibody (NBP1-49887)(1)

Publication using NBP1-49887

• de Bernabe DB, Inamori K, Yoshida-Moriguchi T et al. Loss of alpha-dystroglycan laminin binding in epithelium-derived cancers is caused by silencing of LARGE. J Biol Chem 2009-04-01 [PMID: 19244252]

Bioinformatics

• Gene Symbol: LARGE
• Entrez:
  • 9215(Human)
• Uniprot:
  • NP_004728.1(Human)