LARGE Antibody

Western Blot: LARGE Antibody [NBP1-49865] - Analysis of LARGE (aa421-433) in Human kidney lysate (35 ug protein in RIPA buffer) using NBP1-49865 at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LARGE Antibody Summary

• Description: Novus Biologicals Goat LARGE Antibody (NBP1-49865) is a polyclonal antibody validated for use in WB and ELISA. Anti-LARGE Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SEADVNSENLQKQ corresponding to internal region according to NP_004728.1.
• Epitope: SEADVNSENLQKQ - internal region
• Specificity: Reported variants represent identical protein: NP_004728.1, NP_598397.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LARGE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 75 kDa band observed in human kidney lysate (calculated MW of 88.1 kDa band according to NP_004728.1).

Reactivity Notes

Expected reactivity based on sequence similarity: Bovine, Canine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LARGE Antibody

• Acetylglucosaminyltransferase-like 1A
• acetylglucosaminyltransferase-like protein
• glycosyltransferase-like protein LARGE1
• LARGE
• LARGE1
• like-acetylglucosaminyltransferase
• like-glycosyltransferase
• MDC1D
• MDDGA6
• MDDGB6
• xylosyl- and glucuronyltransferase LARGE1

Background

The LARGE gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LARGE Antibody (NBP1-49865)(1)

Publication using NBP1-49865

• de Bernabe DB, Inamori K, Yoshida-Moriguchi T et al. Loss of alpha-dystroglycan laminin binding in epithelium-derived cancers is caused by silencing of LARGE. J Biol Chem 2009-04-01 [PMID: 19244252]

Bioinformatics

• Gene Symbol: LARGE
• Entrez:
  • 9215(Human)
• Uniprot:
  • NP_004728.1(Human)