Novus Biologicals products are now on bio-techne.com

KvBeta1 Overexpression Lysate

Images

 
Western Blot: KvBeta1.1 Overexpression Lysate (Adult Normal) [NBL1-12141] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KvBeta1.1.
Western Blot: KvBeta1.1 Overexpression Lysate (Adult Normal) [NBL1-12141] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KCNAB1

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KvBeta1 Overexpression Lysate Summary

Description

KvBeta1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207384

Accession#: NM_172159

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
KCNAB1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
44.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KvBeta1 Overexpression Lysate

  • AKR6A3
  • hKvb3
  • hKvBeta3
  • K(+) channel subunit beta-1
  • KCNA1Bpotassium channel beta 3 chain
  • Kvb1.3
  • KV-BETA-1
  • potassium channel beta3 subunit
  • potassium channel shaker chain beta 1a
  • potassium voltage-gated channel beta subunit
  • potassium voltage-gated channel, shaker-related subfamily, beta member 1
  • voltage-gated potassium channel beta-1 subunit
  • voltage-gated potassium channel subunit beta-1

Background

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member includes three distinct isoforms which are encoded by three alternatively spliced transcript variants of this gene. These three isoforms are beta subunits, which form heteromultimeric complex with alpha subunits and modulate the activity of the pore-forming alpha subunits. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-76939
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP3-03729
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
H00003739-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP2-38498
Species: Hu
Applications: IHC, IHC-P
NBP2-83134
Species: Hu
Applications: IHC, IHC-P, WB
NBP3-10293
Species: Hu
Applications: WB
NB110-61010
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IP, RIA, WB
H00030819-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, WB
NBP1-80271
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-22439
Species: Ha, Hu, Mu, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, IP, MiAr, WB
NBP3-03307
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
H00009211-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NBP2-94559
Species: Hu, Mu, Rt
Applications: WB
NBP2-14141
Species: Hu
Applications: IHC, IHC-P
NBP2-85189
Species: Hu
Applications: IHC, IHC-P, WB
NBL1-12141
Species: Hu
Applications: WB

Publications for KvBeta1 Lysate (NBL1-12141) (0)

There are no publications for KvBeta1 Lysate (NBL1-12141).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for KvBeta1 Lysate (NBL1-12141) (0)

There are no reviews for KvBeta1 Lysate (NBL1-12141). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KvBeta1 Lysate (NBL1-12141) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional KvBeta1 Products

Research Areas for KvBeta1 Lysate (NBL1-12141)

Find related products by research area.

Blogs on KvBeta1

There are no specific blogs for KvBeta1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KvBeta1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol KCNAB1