KvBeta1 Overexpression Lysate

Western Blot: KvBeta1.1 Overexpression Lysate (Adult Normal) [NBL1-12141] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KvBeta1.1.

Western Blot: KvBeta1.1 Overexpression Lysate (Adult Normal) [NBL1-12141] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KCNAB1

• Reactivity: Hu
• Applications: WB

KvBeta1 Overexpression Lysate Summary

• Description:

  KvBeta1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC207384
  
  Accession#: NM_172159
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: KCNAB1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 44.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for KvBeta1 Overexpression Lysate

• AKR6A3
• hKvb3
• hKvBeta3
• K(+) channel subunit beta-1
• KCNA1Bpotassium channel beta 3 chain
• Kvb1.3
• KV-BETA-1
• potassium channel beta3 subunit
• potassium channel shaker chain beta 1a
• potassium voltage-gated channel beta subunit
• potassium voltage-gated channel, shaker-related subfamily, beta member 1
• voltage-gated potassium channel beta-1 subunit
• voltage-gated potassium channel subunit beta-1

Background

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member includes three distinct isoforms which are encoded by three alternatively spliced transcript variants of this gene. These three isoforms are beta subunits, which form heteromultimeric complex with alpha subunits and modulate the activity of the pore-forming alpha subunits. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: KCNAB1