Jade-1/PHF17 Antibody

Western Blot: Jade-1/PHF17 Antibody [NBP2-19798] - Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:5000

Immunocytochemistry/Immunofluorescence: Jade-1/PHF17 Antibody [NBP2-19798] - immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PHF17 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: Jade-1/PHF17 Antibody [NBP2-19798] - Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using antibody at 1:500 dilution.

Product Discontinued

Jade-1/PHF17 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PHF17. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Mouse (97%), Rat (99%), Rhesus Macaque (100%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JADE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Jade-1/PHF17 Antibody

• FLJ22479
• Jade1
• Jade-1
• KIAA1807
• PHD finger protein 17JADE1gene for apoptosis and differentiation in epithelia
• PHD protein Jade-1
• PHF17
• protein Jade-1

Background

PHF17 is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activitytoward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator itmay also promote acetylation of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: JADE1
• Uniprot:
  • Q6IE81()