INTS11 Antibody

Images

 
Western Blot: INTS11 Antibody [NB100-60638] - Whole cell lysate (50 ug) from HeLa, K-562, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11antibody used for ...read more
Immunohistochemistry-Paraffin: INTS11 Antibody [NB100-60638] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Biological Strategies: Western Blot: INTS11 Antibody [NB100-60638] - analysis of INTS11 in HeLa whole cell extracts (samples treated with siCtrl or siINTS11) using anti-INTS11 antibody. Image from verified ...read more
Independent Antibodies: Immunoprecipitation: INTS11 Antibody [NB100-60638] - Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm-OrSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

INTS11 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human integrator complex subunit 11 using the numbering given in entry NP_060341.2 (GeneID 54973).
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPSF3L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. INTS11 antibody validated for WB from a verified customer review.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
INTS11 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-60638 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for INTS11 Antibody

  • cleavage and polyadenylation specific factor 3-like
  • Cleavage and polyadenylation-specific factor 3-like protein
  • CPSF3-like protein
  • EC 3.1.27
  • EC 3.1.27.-
  • FLJ20542
  • INT11
  • integrator complex subunit 11
  • INTS11FLJ13294
  • Protein related to CPSF subunits of 68 kDa
  • RC68
  • RC-68CPSF73L
  • related to CPSF subunits 68 kDa

Background

INT11 has been identified as a component of the twelve-subunit integrator complex that functions in RNA Pol II mediated 3'-end mRNA processing of the U1 and U2 small nuclear RNAs (snRNAs). INT11 is a beta-lactamase family member that has sequence similarity to the cleavage and polyadenylation specificity factors CPSF-73 and CPSF-100.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for INTS11 Antibody (NB100-60638) (0)

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Review for INTS11 Antibody (NB100-60638) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-60638:
Filter by Applications
Western Blot
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot INTS11 NB100-60638
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5
reviewed by:
Verified Customer
Western Blot Human 02/13/2015
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Summary

ApplicationWestern Blot
Sample TestedHeLa whole cell extract
SpeciesHuman
LotA1

Product General Protocols

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Video Protocols

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FAQs for INTS11 Antibody (NB100-60638) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for INTS11 Antibody (NB100-60638)

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Recent Reviews

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Verified Customer
02/13/2015
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol CPSF3L
Entrez
Uniprot