![Western Blot: INTS11 Antibody [NB100-60638] - Whole cell lysate (50 ug) from HeLa, K-562, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize2/INTS11-Antibody-Western-Blot-NB100-60638-img0013.jpg "Western Blot: INTS11 Antibody [NB100-60638] - Whole cell lysate (50 ug) from HeLa, K-562, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.")
| Reactivity | Hu, Mu, Pm-OrSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human integrator complex subunit 11 using the numbering given in entry NP_060341.2 (GeneID 54973). |
| Predicted Species | Orangutan (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CPSF3L |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. INTS11 antibody validated for WB from a verified customer review. |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-60638 | Applications | Species |
|---|---|---|
| Cihlarova Z, Kubovciak J, Sobol M et al. BRAT1 links Integrator and defective RNA processing with neurodegeneration Nature communications 2022-08-26 [PMID: 36028512] (WB, Human) Details: Dilutions: 1:1000 |
WB | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Koichi Ogami |
WB | Human | 02/13/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Pathways for INTS11 Antibody (NB100-60638)View related products by pathway.
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PTMs for INTS11 Antibody (NB100-60638)Learn more about PTMs related to INTS11 Antibody (NB100-60638).
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![Immunohistochemistry-Paraffin: INTS11 Antibody [NB100-60638] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize2/INTS11-Antibody-NB100-60638-img0014.jpg "Immunohistochemistry-Paraffin: INTS11 Antibody [NB100-60638] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Western Blot: INTS11 Antibody [NB100-60638] - analysis of INTS11 in HeLa whole cell extracts (samples treated with siCtrl or siINTS11) using anti-INTS11 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/INTS11-Antibody-Western-Blot-NB100-60638-img0004.jpg "Western Blot: INTS11 Antibody [NB100-60638] - analysis of INTS11 in HeLa whole cell extracts (samples treated with siCtrl or siINTS11) using anti-INTS11 antibody. Image from verified customer review.")
![Immunoprecipitation: INTS11 Antibody [NB100-60638] - Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT11 antibody NB100-60638 (lot NB100-60638-3) used for IP at 6 ug per reaction. INT11 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60638) and another rabbit anti-INT11 antibody. For blotting immunoprecipitated INT11, NB100-60638 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/INTS11-Antibody-Immunoprecipitation-NB100-60638-img0012.jpg "Immunoprecipitation: INTS11 Antibody [NB100-60638] - Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT11 antibody NB100-60638 (lot NB100-60638-3) used for IP at 6 ug per reaction. INT11 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60638) and another rabbit anti-INT11 antibody. For blotting immunoprecipitated INT11, NB100-60638 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
