The Quantikine Human KLK5 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human KLK5 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human KLK5 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human KLK5 showed linear curves that were parallel to the standard curves obta...ined using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human KLK5.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human Kallikrein 5 Quantikine ELISA Kit
Kallikrein-like protein 2
kallikrein-related peptidase 5
Stratum corneum tryptic enzyme
The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.
Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.
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