![Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647] - Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein. Lane A: 100ng, Lane B: 20ng. SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-07-Western-Blot-NBP3-06647-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647] - Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein. Lane A: 100ng, Lane B: 20ng. SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
| Reactivity | VSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 07 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HCoV-HKU1 (Accession#: YP_173238.1; Met 1-Arg 760). |
| Specificity | HCoV-HKU1 (isolate N1) Spike/S1 No cross-reactivity in ELISA with: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD Protein SARS-CoV-2 Spike S1 Protein SARS-CoV-2 Spike S1 NTD Protein SARS-CoV-2 Spike S1 NTD Protein SARS-CoV-2 Spike RBD Protein SARS-CoV-2 Spike S2 Protein SARS-CoV-2 Spike S1+S2 ECD Protein SARS-CoV Spike S1+S2 ECD (S577A, Isolate Tor2) Protein MERS-CoV Spike S1+S2 ECD Protein HCoV-NL63 Spike S1+S2 ECD Protein HCoV-229E Spike S1+S2 ECD Protein HCoV-OC43 Spike S1+S2 ECD Protein |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.0) |
| Preservative | 10% Trehalose |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | S |
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")