Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: HBsAg Antibody [NB100-62652] - HepAD38 cells were treated with DFMO (50 uM, 100 uM), and the HBV core-associated DNA was extracted 3 days later and measured by Southern blot (upper panel). The levels of HBc and HBs were measured by Western blotting, and capsids levels were determined using a Native gel assay (lower panel). Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fcimb.2020.00158/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HBsAg-Antibody-Western-Blot-NB100-62652-img0001.jpg "Western Blot: HBsAg Antibody [NB100-62652] - HepAD38 cells were treated with DFMO (50 uM, 100 uM), and the HBV core-associated DNA was extracted 3 days later and measured by Southern blot (upper panel). The levels of HBc and HBs were measured by Western blotting, and capsids levels were determined using a Native gel assay (lower panel). Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fcimb.2020.00158/full), licensed under a CC-BY license.")
![Western Blot: HBsAg Antibody [NB100-62652] - DFMO decreases the HBV core-associated DNA & the HBc protein levels. (A) Determination of cytotoxicity of HepAD38 & HepG2.2.15 cells treated DFMO measured by the MTS assay. (B) HepAD38 cells were treated with DFMO (50 μM, 100 μM), & the HBV core-associated DNA was extracted 3 days later & measured by Southern blot (upper panel). The levels of HBc & HBs were measured by Western blotting, & capsids levels were determined using a Native gel assay (lower panel) as described above. (C) The levels of HBsAg in supernatant were measured by ELISA assay as described above. (D) HBV 3.5kb RNA levels were measured by real-time RT-PCR. (E) & (F) HepG2.2.15 cells treated with DFMO (50, 100 μM) for 3 days, then the levels of intracellular HBV DNA or HBsAg levels in the supernatant were detected by real-time PCR (E) or ELISA assay (F). (G) DFMO decreased the infection capacity of the HBV particles. HepAD38 cells in the absence of tetracycline were treated with DSMO or DFMO with indicated concentration for 3 days, & the HBV viral particles were then collected & added to the HepG2-NTCP cells. Five days later, the cytoplasmic viral DNA were extracted & measured by real-time PCR. RC, relaxed circular; DL, double stranded linear; SS, single stranded. Experiments were performed in triplicate, & data are represented as means ± SD. Statistical significance was determined by one-way ANOVA with the Tukey post-hoc test (*p < 0.05; ns, not significant). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32373551), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-62652_rabbit-polyclonal-hbsag-antibody-31020241553746.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Validation of SARS-Cov-2 Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody NBP3-11940, 0.2-1 ug/mL incubated at RT for 1 hr. Secondary Antibody: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. NBP3-11940 can detect spike S1 protein for all variants except omicron.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0009.jpg "ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Validation of SARS-Cov-2 Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody NBP3-11940, 0.2-1 ug/mL incubated at RT for 1 hr. Secondary Antibody: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. NBP3-11940 can detect spike S1 protein for all variants except omicron.")
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike Antibody [NBP3-11940] - Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike (cleavage site) in 293 Transfected Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Spike transfected 293cells labeling SARS-CoV-2 (COVID-19) Spike (cleavage site) with NBP3-11940 at 10 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11940-img0005.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 Spike, NBP3-11940 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0006.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP3-11940] - Western Blot Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP3-11940-img0007.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - Detection of SARS-CoV-2 UK Variant Spike S1 Protein with Spike S1 Antibodies by Direct ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 ug/mL Incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody, NBP3-11940, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Immunogen region of antibody NBP3-11940 includes site 681P that was mutated in B.1.1.7.. SARS-CoV-2 Spike antibody (NBP3-11940) has lower binding affinity for variant S1 protein as compared to WT S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation of SARS-CoV-2 Spike Antibody Pair. A sandwich ELISA was performed using the anti-SARS-CoV-2 Spike antibodies NBP3-11940 (10 ug/mL ) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies (1 ug/mL ) and streptavidin-HRP (0.1 ug/mL ) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein. Antibodies: SARS-CoV-2 Spike, NBP3-11940. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein, as coating antigen and the anti-SARS-CoV-2 Spike as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody [NBP3-11940] - ELISA Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Antibodies: SARS-CoV-2 Spike antibody, NBP3-11940 (1 ug/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-ELISA-NBP3-11940-img0004.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 125 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/nbp2-90980_human-monoclonal-sars-cov-2-spike-antibody-cr3022-7420252228562.png)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249413310.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024940381.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Inhibition analysis of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (104-13333 pM) using SARS-CoV-2 Neutralizing Antibody ELISA Kit (Omicron BA.1 / BA.2 / BA.4 / BA.5).](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024942542.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249351385.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249355581.jpg)
![Functional (Inhibition): SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 ug/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (1000 ng/mL) binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (0.09-66.67 nM). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (1:10000).](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249374973.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249425449.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024941338.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249415955.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] detects SARS-CoV-2 (COVID-19) Spike RBD Omicron protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike RBD Omicron stained by SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249374937.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.2.12.1 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-2120249393569.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1867) - Omicron Variant - Azide and BSA Free [NBP3-25672] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25672_rabbit-sars-cov-2-spike-rbd-mab-hl1867-omicron-variant-azide-bsa-free-212024940811.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024939162.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024934276.jpg)
![Functional (Inhibition): SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 ug/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (1000 ng/mL) binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (0.09-66.67 nM). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (1:10000).](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249432894.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/ BA.5 Omicron variant; Omicron / XBB variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Please notice that NBP3-25671 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-21202494089.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249413381.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Please notice that NBP3-25671 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249371599.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (NBP3-25672) (1 ug/mL) Please notice that NBP3-25671 and NBP3-25672 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014 and NBP3-25672. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249413317.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag using ELISA pairs below. Pair 1: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL) Pair 2: Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL) Pair 3: Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (HRP) (HL1014-01) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249342714.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.2.12.1 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249374921.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] detects SARS-CoV-2 (COVID-19) Spike RBD Omicron protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike RBD Omicron stained by SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249362729.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP2-41058] - Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. SARS-CoV-2 Spike(1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP2-41058-img0001.jpg "Western Blot: SARS-CoV-2 Spike Antibody [NBP2-41058] - Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. SARS-CoV-2 Spike(1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells")
![Immunohistochemistry-Paraffin: SARS-CoV-2 Spike Antibody [NBP2-41058] - Immunohistochemistry Validation of SARS-CoV-2 Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 Spike S2 antibody (NBP2-41058, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunohistochemistry-Paraffin-NBP2-41058-img0003.jpg)
![Immunohistochemistry-Paraffin: SARS-CoV-2 Spike Antibody [NBP2-41058] - Immunohistochemistry Validation of SARS-CoV-2 Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 Spike S2 antibody (NBP2-41058, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunohistochemistry-Paraffin-NBP2-41058-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - BSA Free [NBP2-41058] - Figure 14 ELISA TestAntibodies: SARS-CoV-2 (COVID-19) Spike antibody, (1 u/mL). A direct ELISA was performed using immunogen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp2-41058_rabbit-polyclonal-sars-cov-2-spike-antibody-271120241958462.jpg)
![Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 Spike Antibody - BSA Free [NBP2-41058] - Validation of SARS-CoV-2 (COVID-19) Spike in 293T Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike with at 1 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp2-41058_rabbit-polyclonal-sars-cov-2-spike-antibody-271120242022975.jpg)
![Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161943.jpg "Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161944.jpg)
![ELISA: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Antibodies: SARS-CoV-2 Spike S1 antibody, NBP3-26918. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike S1 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161940.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in 293 Transfected Cells. Analysis of 4% paraformaldehyde-fixed Spike transfected 293 cells labeling SARS-CoV-2 Spike S1 with NBP3-26918 at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161942.jpg)
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161941.jpg)
![Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of Spike transfected 293 cell lysate. Antibodies: SARS-CoV-2 Spike S2, NBP3-05701, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S2-Antibody-Western-Blot-NBP3-05701-img0004.jpg "Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of Spike transfected 293 cell lysate. Antibodies: SARS-CoV-2 Spike S2, NBP3-05701, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL")
![Immunohistochemistry-Paraffin: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Immunohistochemistry Validation of SARS-CoV-2 Spike S2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S2 antibody (NBP3-05701, 0.1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S2-Antibody-Immunohistochemistry-Paraffin-NBP3-05701-img0002.jpg)
![Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Western Blot Validation with SARS-CoV-2 Spike S2 ECD Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 Spike S2 ECD recombinant protein. Antibodies: SARS-CoV-2 Spike S2, NBP3-05701, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S2-Antibody-Western-Blot-NBP3-05701-img0003.jpg)
![ELISA: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Validation with SARS-CoV-2 Spike S2 ECD Recombinant ProteinSARS-CoV-2 Spike S2 antibody, 9123. A direct ELISA was performed using SARS-CoV-2 Spike S2 ECD recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000ng/mL.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S2-Antibody-ELISA-NBP3-05701-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Recombinant-Protein-ELISA-NBP3-14666-img0001.jpg "ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.")
![ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Recombinant-Protein-ELISA-NBP3-14666-img0002.jpg)
![ELISA: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Recombinant SARS-COV-2 Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-ELISA-NBP2-90985-img0002.jpg "ELISA: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Recombinant SARS-COV-2 Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.")
![HPLC: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - The purity of SARS-COV-2 Spike S1 Protein with His tag (Cat.NBP2-90985) was greater than 95% as determined by SEC-HPLC.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-HPLC-NBP2-90985-img0003.jpg)
![SDS-Page: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Recombinant SARS-COV-2 Spike S1 Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-SDS-Page-NBP2-90985-img0005.jpg)
![Surface Plasmon Resonance: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-Surface-Plasmon-Resonance-NBP2-90985-img0004.jpg)
![SDS-Page: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Recombinant 2019-nCoV Spike RBD Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-SDS-Page-NBP2-90982-img0001.jpg "SDS-Page: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Recombinant 2019-nCoV Spike RBD Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.")
![ELISA: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-ELISA-NBP2-90982-img0002.jpg)
![HPLC: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - The purity of SARS-COV-2 Spike RBD Protein with His tag (Cat.NBP2-90982) was greater than 95% as determined by SEC-HPLC.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-HPLC-NBP2-90982-img0003.jpg)
![Surface Plasmon Resonance: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-Surface-Plasmon-Resonance-NBP2-90982-img0004.jpg)