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![Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-06-Western-Blot-NBP3-14654-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (23) [NBP3-14657] - Anti-Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-23-Western-Blot-NBP3-14657-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (23) [NBP3-14657] - Anti-Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (15) [NBP3-14656] - Anti- Human Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-15-Western-Blot-NBP3-14656-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (15) [NBP3-14656] - Anti- Human Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (11) [NBP3-14653] - Anti- Human Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-11-Western-Blot-NBP3-14653-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (11) [NBP3-14653] - Anti- Human Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (05) [NBP3-14664] - Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-05-Western-Blot-NBP3-14664-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (05) [NBP3-14664] - Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His")
![Western Blot: Human Coronavirus Spike Protein Antibody (29) [NBP3-14658] - Anti-HCoV-OC43 mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-29-Western-Blot-NBP3-14658-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (29) [NBP3-14658] - Anti-HCoV-OC43 mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (10) [NBP3-14652] - Anti- Human Coronavirus Spike Protein Spike mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-10-Western-Blot-NBP3-14652-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (10) [NBP3-14652] - Anti- Human Coronavirus Spike Protein Spike mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647] - Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein. Lane A: 100ng, Lane B: 20ng. SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-07-Western-Blot-NBP3-06647-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647] - Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein. Lane A: 100ng, Lane B: 20ng. SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody [NBP3-12727] - Human coronavirus spike glycoprotein Protein (isolate HKU1) (aa 1-760) Recombinant ProteinLane A: 30ngSecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/1000 dilution.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-Western-Blot-NBP3-12727-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody [NBP3-12727] - Human coronavirus spike glycoprotein Protein (isolate HKU1) (aa 1-760) Recombinant ProteinLane A: 30ngSecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/1000 dilution.Performed under reducing conditions.")