Human Coronavirus Spike Protein Antibody (07) [Alexa Fluor™ Plus 555]

• Reactivity: V
• Applications: WB, ELISA
• Clone: 07
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

Human Coronavirus Spike Protein Antibody (07) [Alexa Fluor™ Plus 555] Summary

• Immunogen: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HCoV-HKU1 (Accession#: YP_173238.1; Met 1-Arg 760).
• Specificity: HCoV-HKU1 (isolate N1) Spike/S1
  
  No cross-reactivity in ELISA with:
  HCoV-HKU1 (isolate N5) Spike S1+S2 ECD Protein
  SARS-CoV-2 Spike S1 Protein
  SARS-CoV-2 Spike S1 NTD Protein
  SARS-CoV-2 Spike S1 NTD Protein
  SARS-CoV-2 Spike RBD Protein
  SARS-CoV-2 Spike S2 Protein
  SARS-CoV-2 Spike S1+S2 ECD Protein
  SARS-CoV Spike S1+S2 ECD (S577A, Isolate Tor2) Protein
  MERS-CoV Spike S1+S2 ECD Protein
  HCoV-NL63 Spike S1+S2 ECD Protein
  HCoV-229E Spike S1+S2 ECD Protein
  HCoV-OC43 Spike S1+S2 ECD Protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

No cross-reactivity in ELISA with:

Human cell lysate (293 cell line)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Human Coronavirus Spike Protein Antibody (07) [Alexa Fluor™ Plus 555]

• HKU1
• Spike glycoprotein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S