HROB Antibody (7A3A9) - BSA Free

Western Blot: C17orf53 Antibody (7A3A9) [NBP2-37407] - Western blot analysis using C17ORF53 mAb against HEK293 (1) and C17ORF53 (AA: 282-527)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: C17orf53 Antibody (7A3A9) [NBP2-37407] - Immunofluorescence analysis of HepG2 cells using C17ORF53 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: C17orf53 Antibody (7A3A9) [NBP2-37407] - Flow cytometric analysis of Jurkat cells using C17ORF53 mouse mAb (green) and negative control (red).

Western Blot: C17orf53 Antibody (7A3A9) [NBP2-37407] - Western blot analysis using C17ORF53 mAb against human C17ORF53 recombinant protein. (Expected MW is 51.9 kDa)

ELISA: C17orf53 Antibody (7A3A9) [NBP2-37407] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: 7A3A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HROB Antibody (7A3A9) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human HROB (AA: 282-527) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: C17orf53
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 69.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HROB Antibody (7A3A9) - BSA Free

• chromosome 17 open reading frame 53
• FLJ11594
• hypothetical protein LOC78995
• MCM8IP
• MGC3130

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HROB Antibody (NBP2-37407)(2)

Publications using NBP2-37407

• Huang JW, Taglialatela A, Acharya A, Leuzzi G Identification of MCM8IP, an interactor of MCM8-9 and RPA1 that promotes homologous recombination and DNA synthesis in response to DNA damage bioRxiv 2019-09-03 (ICC/IF, Human)
• Huang JW, Acharya A, Taglialatela A et al. MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage Nat Commun 2020-06-11 [PMID: 32528060] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: C17orf53