Histone H1.2 Antibody - BSA Free

Western Blot: Histone H1.2 Antibody [NBP2-87570] - WB Suggested Anti-HIST1H1C Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysateHIST1H1C is supported by BioGPS gene expression data to be expressed in 721_B

Immunohistochemistry-Paraffin: Histone H1.2 Antibody [NBP2-87570] - Rabbit Anti-HIST1H1C antibody. Formalin Fixed Paraffin Embedded Tissue: Human Testis. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.

Chromatin Immunoprecipitation: Histone H1.2 Antibody [NBP2-87570] - Chromatin Immunoprecipitation (ChIP) Using HIST1H1C antibody - middle region and HCT116 Cells

Immunohistochemistry-Paraffin: Histone H1.2 Antibody [NBP2-87570] - Rabbit Anti-HIST1H1C antibody. Formalin Fixed Paraffin Embedded Tissue: Human Placenta. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200.

• Reactivity: Hu
• Applications: WB, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Histone H1.2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Histone H1.2 Antibody - BSA Free (NBP2-87570) is a polyclonal antibody validated for use in IHC, WB and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human Histone H1.2. Peptide sequence: ASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKS The peptide sequence for this immunogen was taken from within the described region.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H1-2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Histone H1.2 Antibody - BSA Free

• H1 histone family, member 2
• H1.2
• H1F2H1C
• histone 1, H1c
• histone cluster 1, H1c
• histone H1.2
• Histone H1d
• MGC3992

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The Histone H1.2 protein is 213 amino acids long, 21 kDA and is encoded by the HIST1H1C gene. H1 proteins are essential in forming chromatin fiber and for the condensation of nucleosome chains into higher-order structured fibers. The HIST1H1C gene has been investigated in leukemia and parkinson's disease. It plays a role in apoptosis, PKA signaling, cell cycle chromosome condensation in prometaphase, and histone modification through interactions with various genes such as TONSL, NPM1, YWHAQ, EED, and ESR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-2