Histone H1 Antibody (21NC85)

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Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220] - FFPE pig heart stained with NB100-65220 at 1:500.

Product Details

Summary
Reactivity Hu, Rt, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
21NC85
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Histone H1 Antibody (21NC85) Summary

Immunogen
The exact immunogen is proprietary, but is composed of lysed nuclei of myeloid leukemia biopsy cells.
Marker
Nuclear Marker
Specificity
This antibody recognises an antigen found in the nucleus. Antibody also preferentially reacts with histone H1/ DNA nucleosome as shown by ELISA and western blotting. In WB using non-reduced samples it recognises primarily a 30-32kDa histone H1 band, but other bands are present as well. This antibody stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells. The epitope has not been mapped.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HIST1H1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:20-1:30
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20-1:50
  • Immunohistochemistry-Paraffin 1:500
Application Notes
Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, ELISA and Western Blot
Reviewed Applications
Read 1 Review rated 5
using
NB100-65220 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.1% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Histone H1 Antibody (21NC85)

  • H1 histone family, member 1
  • H1.1
  • H1A
  • H1F1HIST1
  • histone 1, H1a
  • histone cluster 1, H1a
  • histone H1.1
  • MGC126642
  • MGC138345

Background

Histone H1, a nuclear protein necessary for the condensation of nucleosome chains into higher order structures. This antibody stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H1 Antibody (NB100-65220) (0)

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Review for Histone H1 Antibody (NB100-65220) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-65220:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Histone H1 NB100-65220
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reviewed by:
Monika Hilbe
IHC-P Other 10/12/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPig Heart Sections
SpeciesOther
Lot220711

Blocking

Blocking DetailsBlocking Buffer: Peroxidase block with 3% H2O2, Blocking Time: 10 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:500, Incubation Dilution Buffer: PBS pH8, Incubation Time: overnight, Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti Mouse
Secondary Manufacturer Cat#(DAKO) K400511

Details

Detection NotesMethod: AEC, 10-15min under light microscope, Pos.Ctrl: Human cell pellet, Antigen Retrieval: Citrate Buffer pH6, Heat Mediated
Fixation DetailsSample Preparation: paraffin embedded, formalin fixed

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H1 Antibody (NB100-65220). (Showing 1 - 2 of 2 FAQ).

  1. One of our customers injected Human cells into Rabbit tissue. Now he would like to perform IHC to see if Human cells still remain in the Rabbit tissue. The customer searched the Novus website and found NB100-65220. May we ask if NB100-65220 is suitable for such application?
    • I would actually not recommend this antibody to your customer as it is cross reactive to rabbit and human. The customer would require an antibody that only cross reacts with human. If the customer used this antibody they would not be able to differentiate between the human protein or the rabbit protein. I have taken a look for another nuclear antigen antibody however we do not have another one. I am really sorry we don't have what they are looking for.
  2. Do you offer either free samples, or a trial size of this antibody?
    • We do not offer free samples or smaller units of this antibody. As an alternative, we do offer a 100% guarantee for the species and applications listed on the datasheet. If the antibody does not perform as promised, we will be happy to provide a refund or replacement.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NB100-65220

Bioinformatics Tool for Histone H1 Antibody (NB100-65220)

Discover related pathways, diseases and genes to Histone H1 Antibody (NB100-65220). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Histone H1 Antibody (NB100-65220)

Discover more about diseases related to Histone H1 Antibody (NB100-65220).
 

Pathways for Histone H1 Antibody (NB100-65220)

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Blogs on Histone H1.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

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Recent Reviews

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1
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Monika Hilbe
10/12/2011
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol HIST1H1A