HBsAg Antibody

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Western Blot: HBsAg Antibody [NB100-62652] - HepAD38 cells were treated with DFMO (50 uM, 100 uM), and the HBV core-associated DNA was extracted 3 days later and measured by Southern blot (upper panel). The levels of ...read more

Product Details

Summary
Reactivity Hu, Mu, V-ViSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
4.0 mg/ml

Order Details

HBsAg Antibody Summary

Immunogen
Hepatitis B subtypes ad and ay.
Specificity
This product is specific for Hepatitis B surface antigen, recognizing subtypes ad and ay. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans. Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
Publications
Read Publications using
NB100-62652 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
4.0 mg/ml
Purity
Protein A purified

Alternate Names for HBsAg Antibody

  • HBsAg
  • HBV major surface antigen
  • HBV surface antigen
  • Hepatitis B Virus major surface antigen
  • Hepatitis B Virus Surface Antigen
  • Major surface antigen
  • protein S

Background

Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HBsAg Antibody (NB100-62652)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Virus.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Virus
(3)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-62652 Applications Species
Xie H, Xie D, Zhang J et al. ROS/NF-kappa B signaling pathway mediated transcriptional activation of TRIM37 promotes HBV-associated hepatic fibrosis through ubiquitination of SMAD7 Molecular Therapy - Nucleic Acids Aug 1 2020 [PMID: 32916597] (IHC-P, Mouse) IHC-P Mouse
Jeong GU, Ahn BY, Jung J et al. A recombinant human immunoglobulin with coherent avidity to hepatitis B virus surface antigens of various viral genotypes and clinical mutants PLoS ONE Jan 1 2020 [PMID: 32790777] (WB, Human) WB Human
Mao B, Wang Z et al. Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein Levels. Front Cell Infect Microbiol Jul 5 2020 [PMID: 32373551] (WB, Human) WB Human
Chen X, Hu Y, Zhang W et al. Cisplatin induces autophagy to enhance hepatitis B virus replication via activation of ROS/JNK and inhibition of the Akt/mTOR pathway Free Radic. Biol. Med. Dec 11 2018 [PMID: 30550853] (WB, Virus) WB Virus
Dai S, Chen X, Yu Y et al. Immunization with lentiviral vector modified dendritic cells encoding ubiquitinated hepatitis B core antigen promotes Th1 differentiation and antiviral immunity by enhancing p38 MAPK and JNK activation in HBV transgenic mice. Mol Med Rep Sep 14 2018 [PMID: 30221736] (IHC, Virus) IHC Virus
Li X, Pan E, Zhu J, Xu L. Cisplatin Enhances Hepatitis B Virus Replication and PGC-1a Expression through Endoplasmic Reticulum Stress. Sci Rep. 2018 Feb 22 [PMID: 29472690] (Human) Human
Shen Z, Yang H, Yang S et al. Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance. Nat Commun 2017 Dec 14 [PMID: 29242561] (WB, Mouse) WB Mouse
Zhou H, Gewaily D, Ahn SH et al. Sequence analysis and functional characterization of full-length hepatitis B virus genomes from Korean cirrhotic patients with or without liver cancer. Virus Res. Apr 1 2017 [PMID: 28373061] (Virus) Virus
Li J, Zong L, Sureau C et al. UNUSUAL FEATURES OF SODIUM TAUROCHOLATE COTRANSPORTING POLYPEPTIDE AS A HEPATITIS B VIRUS RECEPTOR J. Virol. 2016 Jul 06 [PMID: 27384660]

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HBsAg Antibody (NB100-62652). (Showing 1 - 2 of 2 FAQs).

  1. Our customer would like to know if NB100-62652 would recognize pre-S1 protein. If it is confirmed that this HBsAg antibody would bind to pre-S1, would you please help check if any HBsAg antibody provided would NOT recognize pre-S1?
    • The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119 amino acid region called preS1. Now, all of our HBsAg antibodies are generated against native HBsAg molecule from various subtypes and I do not see any antibody which would not recognize pre-S1.
  2. What is the immunogen used to generate this antibody?
    • The immunogen was the whole organism, and we don't have any epitope information available. Since this antibody NB100-62652 (HBsAg Antibody) is a polyclonal one, I anticipate that the surface of HBsAg will be covered by the IgG molecules.

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Bioinformatics

Gene Symbol S