Western Blot: HBsAg Antibody [NB100-62652] - HepAD38 cells were treated with DFMO (50 uM, 100 uM), and the HBV core-associated DNA was extracted 3 days later and measured by Southern blot (upper panel). The levels of ...read more
This product is specific for Hepatitis B surface antigen, recognizing subtypes ad and ay. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans. Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
Protein A purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Protein A purified
Alternate Names for HBsAg Antibody
HBV major surface antigen
HBV surface antigen
Hepatitis B Virus major surface antigen
Hepatitis B Virus Surface Antigen
Major surface antigen
Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for HBsAg Antibody (NB100-62652)(9)
We have publications tested in 3 confirmed species: Human, Mouse, Virus.
We have publications tested in 3 applications: IHC, IHC-P, WB.
FAQs for HBsAg Antibody (NB100-62652). (Showing 1 - 2 of 2 FAQs).
Our customer would like to know if NB100-62652 would recognize pre-S1 protein. If it is confirmed that this HBsAg antibody would bind to pre-S1, would you please help check if any HBsAg antibody provided would NOT recognize pre-S1?
The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119 amino acid region called preS1. Now, all of our HBsAg antibodies are generated against native HBsAg molecule from various subtypes and I do not see any antibody which would not recognize pre-S1.
What is the immunogen used to generate this antibody?
The immunogen was the whole organism, and we don't have any epitope information available. Since this antibody NB100-62652 (HBsAg Antibody) is a polyclonal one, I anticipate that the surface of HBsAg will be covered by the IgG molecules.
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